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Respiration buffer tablets are for use with the Glycolysis Stress Test Complete Assay Kit (ab222946), Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945).

Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. ab222946 combines Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945) in one practical kit for single purchase.

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Key facts

Form
Solid

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Respiration buffer tablets are for use with the Glycolysis Stress Test Complete Assay Kit (ab222946), Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945).

Glycolysis Stress Test Complete Assay Kit (ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. ab222946 combines Glycolysis Assay [Extracellular acidification] (ab197244) and Glycolysis Stress Test Companion Assay (ab222945) in one practical kit for single purchase.

Key facts

Form
Solid
Storage buffer

Constituents: 35.42% Sodium chloride, 31.48% Potassium chloride, 28.33% Glucose, 2.75% Calcium Chloride Dihydrate, 0.76% Magnesium sulphate, 0.71% Dibasic monohydrogen potassium phosphate, 0.54% Potassium phosphate monobasic

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
+4°C

Notes

Respiration buffer tablets are for use with the Glycolysis Stress Test Complete Assay Kit (Glycolysis Stress Test Complete Assay Kit ab222946), Glycolysis Assay [Extracellular acidification] (Glycolysis Assay [Extracellular acidification] ab197244) and Glycolysis Stress Test Companion Assay (Glycolysis Stress Test Companion Assay ab222945).

Glycolysis Stress Test Complete Assay Kit (Glycolysis Stress Test Complete Assay Kit ab222946) offers a complete solution to measure glycolytic characterization at user-defined glucose concentrations in live cells using conventional fluorescence plate readers. Glycolysis Stress Test Complete Assay Kit ab222946 combines Glycolysis Assay [Extracellular acidification] (Glycolysis Assay [Extracellular acidification] ab197244) and Glycolysis Stress Test Companion Assay (Glycolysis Stress Test Companion Assay ab222945) in one practical kit for single purchase.

Reconstituting this tablet gives you the low buffering media used in the Glycolysis assay.

Reconstitute a Respiration Buffer tablet in 50 mL of water, warm to assay temperature (37 °C), adjust pH to approx. pH 7.4, and filter sterilize using a 0.22 μm filter

The dry material should be stored at room temperature and the reconstituted and filter sterilized product can be stored between +2 to +8 °C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    For this product, it's our understanding that no specific protocols are required. You can:

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