AB327075
SuperNova Staining Buffer (ab327075) has been designed to prevent non-specific interactions between SuperNova antibody conjugates when they are combined in a staining cocktail for flow cytometry experiments.
- Prevents non-specific interactions between SuperNova polymer antibody conjugates
- Compatible with other fluorochrome-conjugated antibodies
- Suitable for both surface or intracellular stanning protocols
- Prevents non-specific interactions between SuperNova polymer antibody conjugates
- Compatible with other fluorochrome-conjugated antibodies
- Suitable for both surface or intracellular stanning protocols
1 Images
- Flow Cyt
Supplier Data
Flow Cytometry - SuperNova Staining Buffer (AB327075)
Example Data : The dual-parameter fluorescence dot plots below illustrate the staining obtained on a normal K3EDTA anti-coagulated blood sample incubated with a cocktail of three SuperNova antibody conjugates with or without co-addition of the SuperNova Staining Buffer. Acquisition is with a CytoFLEX flow cytometer, using CytExpert acquisition software. Analysis is with Kaluza analysis software.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
The SuperNova Staining Buffer (ab327075) has been designed to prevent non-specific interactions between SuperNova antibody conjugates when they are combined in a staining cocktail for flow cytometry experiments. The buffer is only required when the staining cocktail contains more than one SuperNova/polymer dye antibody conjugate.
Abnormal staining may occur if the SuperNova Staining Buffer is omitted or used incorrectly, and data may appear under-compensated. The SuperNova Staining Buffer is compatible with other fluorochrome-conjugated antibodies and can be used for both surface and intracellular staining protocols
Procedure
• We recommend using 10 µL of SuperNova Staining Buffer per 100 µL of sample.
• Add SuperNova Staining Buffer to the test tube or plate first, prior to adding any antibody conjugates or biological samples.
• Once the buffer has been added, follow the standard flow cytometry staining protocol appropriate for the sample type and application.
• For convenience, an antibody staining cocktail master mix may be prepared by incorporating SuperNova Staining Buffer into the staining mixture.
• Pre-mixing and storing SuperNova Staining Buffer with antibody conjugates is not recommended, as non-specific interactions between antibody conjugates may occur over time and adversely affect staining performance.
Abnormal staining may occur if the SuperNova Staining Buffer is omitted or used incorrectly, and data may appear under-compensated. The SuperNova Staining Buffer is compatible with other fluorochrome-conjugated antibodies and can be used for both surface and intracellular staining protocols
Procedure
• We recommend using 10 µL of SuperNova Staining Buffer per 100 µL of sample.
• Add SuperNova Staining Buffer to the test tube or plate first, prior to adding any antibody conjugates or biological samples.
• Once the buffer has been added, follow the standard flow cytometry staining protocol appropriate for the sample type and application.
• For convenience, an antibody staining cocktail master mix may be prepared by incorporating SuperNova Staining Buffer into the staining mixture.
• Pre-mixing and storing SuperNova Staining Buffer with antibody conjugates is not recommended, as non-specific interactions between antibody conjugates may occur over time and adversely affect staining performance.
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Target data
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