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AATF KO cell line available to order. Free of charge wild type control provided.

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Key facts

Cell type

PC-3

Species or organism

Human

Tissue

Prostate

Form

Liquid

Alternative names

Recommended products

AATF KO cell line available to order. Free of charge wild type control provided.

Key facts

Cell type

PC-3

Form

Liquid

Disease

Adenocarcinoma

Concentration
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Properties

Gene name

AATF

Gene editing type

Knockout

Gene editing method

CRISPR technology

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AATF also known as apoptosis antagonizing transcription factor and sometimes referred to as DED a.k.a. death effector detach protein is a pivotal regulator of cellular processes. This protein has a molecular mass of approximately 61 kDa. AATF is expressed in various tissues including the brain and liver where it plays a diverse role in cellular function. As an antagonist of apoptosis AATF interacts with signaling pathways to modulate cell survival and proliferation.

Biological function summary

The protein is involved in numerous cellular activities such as transcriptional regulation and cell cycle control. AATF associates with nucleoprotein complexes and is critical in regulating transcription by interacting with RNA polymerase II. It acts as a transcriptional co-regulator influencing genes responsible for cell growth and survival. Research indicates that AATF can affect the balance between cell survival and death which is significant in both development and response to cellular stress.

Pathways

AATF interacts with multiple signaling cascades notably the apoptosis and cell cycle pathways. For example AATF participates in the p53 pathway influencing cellular responses to DNA damage. It interacts with other proteins such as p21 and cyclin D1 helping to modulate cell cycle progression and ensuring genomic stability. These interactions highlight AATF's role in maintaining cellular homeostasis and regulating programmed cell death processes.

Associated diseases and disorders

AATF is associated with cancer and neurodegenerative diseases. Its ability to inhibit apoptosis and regulate cell proliferation links it to tumorigenesis. In cancer AATF's interaction with proteins like Bcl-2 can prevent the initiation of apoptosis contributing to tumor cell survival. Additionally imbalance in AATF activity may influence the progression of neurodegenerative conditions where its regulatory role on apoptosis and cell cycle can affect neuronal survival and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com