Human ABCC1 knockout MCF7 cell line
Be the first to review this product! Submit a review
|
(0 Publication)
ABCC1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. knockout.
View Alternative Names
ABC 29, ABCC, ABCC 1, ATP binding cassette transporter variant ABCC1delta ex13, ATP binding cassette transporter variant ABCC1delta ex13&14, ATP binding cassette transporter variant ABCC1delta ex25, ATP binding cassette transporter variant ABCC1delta ex25&26, ATP binding cassette, sub-family C (CFTR/MRP), member 1, ATP-binding cassette sub-family C member 1, DKFZp686N04233, DKFZp781G125, GS X, LTC4 transporter, Leukotriene C(4) transporter, MRP1_HUMAN, Multidrug resistance protein, Multidrug resistance-associated protein 1, Multiple drug resistance associated protein, Multiple drug resistance protein 1
- WB
Lab
Western blot - Human ABCC1 knockout MCF7 cell line (AB286378)
Western blot : Rabbit Monoclonal [EPR21062] to MRP1 ab233383 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta.
A band was observed at 172 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in abCC1 knockout MCF7 cell line.
To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-MRP1 antibody [EPR21062] (<a href='/en-us/products/primary-antibodies/mrp1-antibody-epr21062-ab233383'>ab233383</a>) at 1/1000 dilution
Lane 1:
Wild-type MCF7 at 20 µg
Lane 2:
ABCC1 knockout MCF7 at 20 µg
Lane 2:
Western blot - Human ABCC1 knockout MCF7 cell line (ab286378) at 20 µg
Lane 3:
DU 145 at 20 µg
Lane 4:
Ramos at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 172 kDa
Observed band size: 172 kDa
false
- WB
Lab
Western blot - Human ABCC1 knockout MCF7 cell line (AB286378)
Western blot : Rabbit Monoclonal [EPR22841-78] to MRP1 ab260038 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta.
A band was observed at 172 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in abCC1 knockout MCF7 cell line.
To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-MRP1 antibody [EPR22841-78] (<a href='/en-us/products/primary-antibodies/mrp1-antibody-epr22841-78-ab260038'>ab260038</a>) at 1/1000 dilution
Lane 1:
Wild-type MCF7 at 20 µg
Lane 2:
ABCC1 knockout MCF7 at 20 µg
Lane 2:
Western blot - Human ABCC1 knockout MCF7 cell line (ab286378) at 20 µg
Lane 3:
DU 145 at 20 µg
Lane 4:
Ramos at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 172 kDa
Observed band size: 172 kDa
false
- WB
Lab
Western blot - Human ABCC1 knockout MCF7 cell line (AB286378)
Western blot : Rabbit Monoclonal [EPR21061] to MRP1 ab230948 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta.
A band was observed at 172 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in abCC1 knockout MCF7 cell line.
To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-MRP1 antibody [EPR21061] (<a href='/en-us/products/primary-antibodies/mrp1-antibody-epr21061-ab230948'>ab230948</a>) at 1/1000 dilution
Lane 1:
Wild-type MCF7 at 20 µg
Lane 2:
ABCC1 knockout MCF7 at 20 µg
Lane 2:
Western blot - Human ABCC1 knockout MCF7 cell line (ab286378) at 20 µg
Lane 3:
DU 145 at 20 µg
Lane 4:
Ramos at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 172 kDa
Observed band size: 172 kDa
false
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type MCF7 cell line (ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
MEM + 10% FBS + 0.01 mg/ml bovine insulin
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein functions as an important determinant of multidrug resistance in cancer treatment. MRP1 can form part of a larger protein complex that includes other transporters like MRP2. Through its activity MRP1 protects tissues from toxic substances by transporting them out of the cell. Its role in transporting glutathione-conjugated compounds highlights its importance in cellular defense mechanisms against oxidative stress and xenobiotics.
Pathways
MRP1 participates in the glutathione metabolism and the xenobiotic efflux pathways. Both pathways involve cellular detoxification and the accumulation of anomalies can cause harmful effects in the body. MRP1 works with proteins such as GSTP1 which conjugates toxic substances with glutathione preparing them for export by MRP1. This coordination ensures efficient detoxification and protection of cells from damage.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Cell Lines & Lysates
AB255371
Human CAV1 (Caveolin-1) knockout HeLa cell line
Advanced Validation
- 0
- 0
![Western blot - Anti-MRP1 antibody [EPR22841-78] (AB260038)](https://content.abcam.com/products/images/mrp1-antibody-epr22841-78-ab260038--western-blot-img447433.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com