Human ACAD9 knockout HeLa cell line
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ACAD9 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
ACAD9_HUMAN, Acyl CoA dehydrogenase 9, Acyl Coenzyme A dehydrogenase family, member 9, Acyl-CoA dehydrogenase family member 9, FLJ23533, MGC14452, NPD002, Very long chain acyl CoA dehydrogenase VLCAD, acyl-CoA dehydrogenase family member 9, mitochondrial, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human ACAD9 knockout HeLa cell line (AB265168)
Homozygous : 1 bp insertion in exon 1.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ACAD9 participates in key enzymatic activities important for energy production. It forms part of a larger enzyme complex known as the electron transfer flavoprotein (ETF)-ubiquinone oxidoreductase complex which is essential for the transfer of electrons in the mitochondrial respiratory chain. This protein aids in converting stored fatty acids into acetyl-CoA which is then funneled into the Krebs cycle for further energy generation.
Pathways
ACAD9 is intricately linked to mitochondrial fatty acid oxidation and energy metabolism. It plays a central role in the β-oxidation pathway which is critical for converting fatty acids into usable energy. ACAD9 interacts with related proteins such as electron transfer flavoprotein (ETF) and ETF-ubiquinone oxidoreductase facilitating the transfer of electrons derived from fatty acid metabolism into the respiratory chain thereby producing ATP efficiently.
Quality control
STR analysis
TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820, D5S818
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com