Human ACE2 knockout Caco-2 cell line (ab273731)

ACE2 KO cell line available now. KO validated by Immunocytochemistry, Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 62 bp deletion in exon 2.

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Alternative names=ACE-related carboxypeptidase, ACE2_HUMAN, ACEH, APC1 protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, Angiotensin converting enzyme 2, Angiotensin converting enzyme like protein, Angiotensin-converting enzyme homolog, B cell differentiation factor, B-cell stimulatory factor 2, BSF-2, C17orf33, CSF beta, CSF3OS, CSF3_HUMAN, CTL differentiation factor, Cachectin, Colony stimulating factor 3, Colony stimulating factor 3 (granulocyte), Csfg, Cytotoxic T cell differentiation factor, D16Ertd61e, DIF, DKFZP434A014, Differentiation inducing factor, EC 3.4.17, Epitheliasin, FLJ41954, Filgrastim, G-CSF, GCSA, Granulocyte colony-stimulating factor, HSF, Hepatocyte stimulating factor, Hepatocyte stimulatory factor, Hybridoma growth factor, Hybridoma growth factor Interferon beta-2, Hybridoma plasmacytoma growth factor, IFN-beta-2, IFNB2, IL6_HUMAN, Interferon beta-2, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, Lenograstim, MGC45931, MGC6821, MGI 2, Macrophage cytotoxic factor, Macrophage granulocyte inducer 2, OTTHUMP00000022963, PP9284, PRSS10, Pluripoietin, Processed angiotensin-converting enzyme 2, Serine protease 10, TMPRSS2, TMPRSS2 ERG FUSION GENE, INCLUDED, TMPRSS2 ETV1 FUSION GENE, INCLUDED, TMPS2_HUMAN, TNF superfamily member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-alpha, TNFA_HUMAN, TNFSF2, Tnf, Transmembrane protease serine 2 catalytic chain, Transmembrane protease, serine 2, Transmembrane protease, serine 2, EC 3.4.219, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2), Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, soluble form, metalloprotease MPROT 15

Cell type: Caco-2
Species or organism: Human
Tissue: Colon
Form: Liquid
Knockout validation: Immunocytochemistry, Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 62 bp deletion in exon 2

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Key facts

Cell type: Caco-2
Species or organism: Human
Tissue: Colon
Form: Liquid
Knockout validation: Immunocytochemistry, Sanger Sequencing, Western blot
Mutation description: Knockout achieved by using CRISPR/Cas9, Homozygous: 62 bp deletion in exon 2
Concentration

Properties

Gene name: ACE2
Gene editing type: Knockout
Gene editing method: CRISPR technology
Knockout validation: Immunocytochemistry, Sanger Sequencing, Western blot
Zygosity: Homozygous

Quality control

STR analysis: CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level: EU: 1 US: 1
Adherent/suspension: Adherent
Gender: Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO₂. Check the culture one day after revival and continue to check until 70% confluent.
5. Once confluent passage into an appropriate flask at a density of 2x10⁴ cells/cm². Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

Do not allow cells to become more than 70% confluent as they will form vacuoles in the cytoplasm.

Slow to trypsinise, however, prolonged trypsinisation is detrimental to cell viability. Trypsinise until 70% of cells have detached.

All seeding densities should be based on cell counts gained by established methods.

A guide seeding density of 1x10⁴ - 2x10⁴ cells/cm² is recommended.

Culture medium

EMEM + 20% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions: Dry Ice
Appropriate long-term storage conditions: -196°C

Notes

Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture medium: EMEM + 20% FBS

Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 1x10⁴ cells/cm². Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO₂. Cultures should be monitored daily.

Subculture guidelines:

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 1x10⁴ cells/cm² is recommended.
A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.
Cells should be passaged when they have achieved 80-90% confluence (approx 8x10⁴-1x10⁵ cells/cm²).

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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Human ACE2 knockout Caco-2 cell line

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