Human ACP1 (Acid phosphatase) knockout HEK-293 cell line
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ACP1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%.
View Alternative Names
ACP1, Acid phosphatase 1 soluble, Acid phosphatase of erythrocyte, Adipocyte acid phosphatase, Cytoplasmic phosphotyrosyl protein phosphatase, HAAP, LMW-PTP, LMW-PTPase, Low molecular weight cytosolic acid phosphatase, Low molecular weight phosphotyrosine protein phosphatase, PAP1, PAP2, PPAC_HUMAN, PTPase, Protein tyrosine phosphatase, Purple acid phosphatase, Red cell acid phosphatase 1, phosphatase, acid, of erythrocyte, testicular secretory protein Li 37
- WB
Lab
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)
Lanes 1 - 4 : Merged signal (red and green). Green - ab235448 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab235448 was shown to specifically react with ACP1 (Acid phosphatase 1) in wild-type HEK 293 cells as signal was lost in ACP1 knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab235448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4° at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Acid phosphatase antibody [EPR21787] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-antibody-epr21787-ab235448'>ab235448</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (ab261859)
Lane 3:
K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg
Lane 4:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Predicted band size: 18 kDa
false
- WB
Lab
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)
Lanes 1 - 4 : Merged signal (red and green). Green - ab166896 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab166896 was shown to recognize ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost at the expected MW in ACP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab166896 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4° at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Acid phosphatase/ACP1 antibody [EPR9839] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-acp1-antibody-epr9839-ab166896'>ab166896</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (ab261859)
Lane 3:
K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg
Lane 4:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Predicted band size: 18 kDa
Observed band size: 18 kDa
false
- WB
Lab
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180524 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180524 was shown to specifically react with ACP1 (Acid phosphatase 1) in wild-type HEK-293 cells as signal was lost in ACP1 knockout cell line ab261859 (knockout cell lysate ab261668). Wild-type and ACP1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab180524 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-Acid phosphatase/ACP1 antibody [EPR9838(2)] (<a href='/en-us/products/unavailable/acid-phosphataseacp1-antibody-epr98382-ab180524'>ab180524</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 whole cell lysate at 20 µg
Lane 2:
ACP1 knockout HEK-293 whole cell lysate at 20 µg
Lane 2:
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (ab261859)
Lane 3:
K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg
Lane 4:
HeLa whole cell lysate at 20 µg
false
- WB
Lab
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)
Lanes 1 - 4 : Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout sample. Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab235449 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Acid phosphatase antibody [EPR21791] (<a href='/en-us/products/primary-antibodies/acid-phosphatase-antibody-epr21791-ab235449'>ab235449</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (ab261859)
Lane 3:
K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg
Lane 4:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Predicted band size: 18 kDa
Observed band size: 18 kDa
false
- NGS
Lab
Next Generation Sequencing - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (AB261859)
X = 1 bp insertion
Reactivity data
Product details
Recommended control: Human wild-type HEK-293 cell line (ab259776). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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