ADARB1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5
Alternative names
1700057H01Rik, ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB 1, AW124433, AW558573, Adenosine deaminase, RNA specific, 2, Adenosine deaminase, RNA specific, B1, Adenosine deaminase, RNA specific, B1 (RED1 homolog rat), Adenosine deaminase, RNA specific, B1 (homolog of rat RED1), BB220382, D10Bwg0447e, DRABA2, DRADA2, Double-stranded RNA-specific editase 1, EC 3.5.-.-, Human dsRNA adenosine deaminase DRADA2, Human dsRNA adenosine deaminase DRADA2b, EC 3.5, OTTHUMP00000115341, OTTHUMP00000115342, RED1_HUMAN, RNA editase, RNA editase 1, RNA editing enzyme 1, rat, homolog of, RNA specific adenosine deaminase B1, RNA-editing deaminase 1, RNA-editing enzyme 1, dsRNA adenosine deaminase
Recommended products
ADARB1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5
- Concentration
Properties
- Gene name
- ADARB1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RED1 also known as ADARB1 is a protein involved in RNA editing. It weighs approximately 73 kDa. RED1 mainly expresses itself in the central nervous system but also appears in other tissues. This protein modifies RNA molecules by catalyzing the conversion of adenosine to inosine which can alter the coding potential and metabolism of the RNA it acts upon.
- Biological function summary
The protein RED1 functions within RNA editing complexes. It co-operates with other proteins like ADAR1 to carry out its editing role. In particular RED1 affects the regulation of gene expression influencing neuronal function and development in the brain. Through its editing capabilities the protein contributes to the diversity and stability of RNA transcripts.
- Pathways
RED1 plays a role in neurotransmission and neurodevelopment pathways. It interacts with other editing proteins in the ADAR family to ensure proper RNA sequence modifications critical for brain function. The editing process that RED1 participates in impacts synaptic transmission important for neuronal communication and neural network formation.
- Associated diseases and disorders
The protein RED1 is associated with neurological disorders. Changes in RED1 expression or mutations can link to conditions such as epilepsy and schizophrenia. Aberrations in RNA editing performed by RED1 might lead to the dysfunction of proteins involved in neuronal signaling. It also connects with proteins like ADAR2 influencing the pathological progress of these conditions.
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1 product image
Sanger Sequencing - Human ADARB1 (RED1) knockout HEK-293T cell line (ab267278)
Homozygous: 1 bp insertion in exon5
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