Human AGO1 knockout HeLa cell line
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- Cell Culture
Unknown
Cell Culture - Human AGO1 knockout HeLa cell line (AB265481)
Representative images of AGO1 knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.
- Sanger seq
Unknown
Sanger Sequencing - Human AGO1 knockout HeLa cell line (AB265481)
Allele-1 : 1 bp deletion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human AGO1 knockout HeLa cell line (AB265481)
Allele-2 : 1 bp insertion in exon 2.
Complementary Products
Primary Antibodies
AB300153
Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free)
RabMAb|Recombinant
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--immunoprecipitation-img165734.jpg)
- 0
- 0
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ago1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.
Pathways
The functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com