Human ALDH1A1 knockout A549 cell line
- Advanced Validation
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ALDH1A1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 8 bp deletion Frameshift: 98%.
View Alternative Names
AHD2, AL1A1_HUMAN, ALDC, ALDH 1, ALDH 11, ALDH class 1, ALDH, liver cytosolic, ALDH-E1, ALHDII, Acetaldehyde dehydrogenase 1, Aldehyde dehydrogenase, Aldehyde dehydrogenase 1 family member A1, Aldehyde dehydrogenase 1 soluble, Aldehyde dehydrogenase 1A1, Aldehyde dehydrogenase cytosolic, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase liver cytosolic, HEL-S-53e, MGC2318, PUMB 1, RALDH 1, Retinal dehydrogenase 1, cytosolic, epididymis luminal protein 12, epididymis luminal protein 9, epididymis secretory sperm binding protein Li 53e
- WB
Lab
Western blot - Human ALDH1A1 knockout A549 cell line (AB261864)
All lanes:
Western blot - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (<a href='/en-us/products/primary-antibodies/aldh1a1-antibody-ep1933y-c-terminal-ab52492'>ab52492</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ALDH1A1 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ALDH1A1 knockout A549 cell line (ab261864)
Lane 2:
Western blot - Human ALDH1A1 knockout A549 cell lysate (<a href='/en-us/products/cell-lysates/human-aldh1a1-knockout-a549-cell-lysate-ab261673'>ab261673</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
false
- WB
Lab
Western blot - Human ALDH1A1 knockout A549 cell line (AB261864)
All lanes:
Western blot - Anti-ALDH1A1 antibody [ALDH1A1/1381] (<a href='/en-us/products/primary-antibodies/aldh1a1-antibody-aldh1a1-1381-ab219303'>ab219303</a>) at 1 µg/mL
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ALDH1A1 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ALDH1A1 knockout A549 cell line (ab261864)
Lane 2:
Western blot - Human ALDH1A1 knockout A549 cell lysate (<a href='/en-us/products/cell-lysates/human-aldh1a1-knockout-a549-cell-lysate-ab261673'>ab261673</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
MCF7 cell lysate at 20 µg
false
- NGS
Supplier Data
Next Generation Sequencing - Human ALDH1A1 knockout A549 cell line (AB261864)
Knockout achieved by CRISPR/Cas9; X = 8 bp deletion; Frameshift : 98%
Reactivity data
Product details
Recommended control: Human wild-type A549 cell line (ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.
Pathways
The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1's role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB52492
Anti-ALDH1A1 antibody [EP1933Y] - C-terminal
RabMAb|Recombinant|KO Validated|20ul selling size
![Flow Cytometry (Intracellular) - Anti-ALDH1A1 antibody [EP1933Y] - C-terminal (AB52492)](https://content.abcam.com/products/images/aldh1a1-antibody-ep1933y-c-terminal-ab52492--flow-cytometry-intracellular-img89276.jpg)
- 4
- 22
Biochemicals
AB141406
PDTC (Ammonium pyrrolidinedithiocarbamate), NF-kappaB inhibitor antioxidant
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com