Human AP1M1 knockout HeLa cell line
Be the first to review this product! Submit a review
|
(0 Publication)
AP1M1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon 1 and 26 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
AP-1 complex subunit mu-1, AP-mu chain family member mu1A, AP1M1_HUMAN, AP47, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, CLAPM2, CLTNM, Clathrin adaptor protein AP47, Clathrin assembly protein complex 1 medium chain, Clathrin assembly protein complex 1 medium chain 1, Clathrin assembly protein complex AP1 mu subunit, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor AP 1 47 kDa protein, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, HA1 47 kDa subunit, MU 1A, Mu-adaptin 1, Mu1A-adaptin
- Sanger seq
Unknown
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (AB265506)
Allele-3 : Insertion of the selection cassette in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (AB265506)
Allele-1 : 25 bp insertion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (AB265506)
Allele-2 : 26 bp insertion in exon 1.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AP1M1 serves as a critical link in intracellular transport processes. It is part of the AP-1 complex which consists of other subunits like gamma beta and sigma. These complexes work together to ensure proteins move correctly within the cell especially between the trans-Golgi network and endosomes. AP1M1 assists in clathrin-mediated trafficking playing an important role in maintaining cellular functionality by organizing the delivery of membrane proteins and other cellular components.
Pathways
AP1M1 integrates into the clathrin-coated vesicle pathway and the endocytic pathway. It cooperates with proteins such as clathrin and adaptins including other AP complexes to facilitate the formation of transport vesicles. These pathways are pivotal for protein sorting and the regulation of cellular homeostasis contributing to processes such as receptor recycling and lysosomal enzyme targeting.
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com