AP1M1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon 1 and 26 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon 1 and 26 bp insertion in exon 1 and Insertion of the selection cassette in exon 1
Alternative names
AP-1 complex subunit mu-1, AP-mu chain family member mu1A, AP1M1_HUMAN, AP47, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, CLAPM2, CLTNM, Clathrin adaptor protein AP47, Clathrin assembly protein complex 1 medium chain, Clathrin assembly protein complex 1 medium chain 1, Clathrin assembly protein complex AP1 mu subunit, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor AP 1 47 kDa protein, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, HA1 47 kDa subunit, MU 1A, Mu-adaptin 1, Mu1A-adaptin
Recommended products
AP1M1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon 1 and 26 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 25 bp insertion in exon 1 and 26 bp insertion in exon 1 and Insertion of the selection cassette in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- AP1M1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AP1M1 also known as Adaptor-related Protein Complex 1 Mu 1 Subunit is a protein with a molecular mass of around 50 kDa. AP1M1 functions as a component of the adaptor protein complex 1 (AP-1). It plays an essential mechanical role in sorting and trafficking proteins by mediating the selection of cargo molecules for transport vesicles. This protein is expressed in multiple tissues with particularly high levels noted in neuronal cells.
- Biological function summary
AP1M1 serves as a critical link in intracellular transport processes. It is part of the AP-1 complex which consists of other subunits like gamma beta and sigma. These complexes work together to ensure proteins move correctly within the cell especially between the trans-Golgi network and endosomes. AP1M1 assists in clathrin-mediated trafficking playing an important role in maintaining cellular functionality by organizing the delivery of membrane proteins and other cellular components.
- Pathways
AP1M1 integrates into the clathrin-coated vesicle pathway and the endocytic pathway. It cooperates with proteins such as clathrin and adaptins including other AP complexes to facilitate the formation of transport vesicles. These pathways are pivotal for protein sorting and the regulation of cellular homeostasis contributing to processes such as receptor recycling and lysosomal enzyme targeting.
- Associated diseases and disorders
Mutations or dysfunction in AP1M1 are linked with neurodegenerative diseases like Alzheimer's disease and some cancers. AP1M1's interaction with proteins like tau in Alzheimer’s illustrates its importance in neurological pathways and synaptic health. In cancer improper protein sorting due to AP1M1 malfunction may influence metastatic behavior as it is connected to proteins involved in cell growth and division.
Product promise
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3 product images
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (ab265506)
Allele-3: Insertion of the selection cassette in exon 1.
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (ab265506)
Allele-1: 25 bp insertion in exon 1.
Sanger Sequencing - Human AP1M1 knockout HeLa cell line (ab265506)
Allele-2: 26 bp insertion in exon 1.
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