APOE KO cell line available to order. Free of charge wild type control provided.
Images
Publications
Filter by
- Nature microbiology 9:1499-15122024Crimean-Congo haemorrhagic fever virus uses LDLR to bind and enter host cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa M Monteil,Shane C Wright,Matheus Dyczynski,Max J Kellner,Sofia Appelberg,Sebastian W Platzer,Ahmed Ibrahim,Hyesoo Kwon,Ioannis Pittarokoilis,Mattia Mirandola,Georg Michlits,Stephanie Devignot,Elizabeth Elder,Samir Abdurahman,Sándor Bereczky,Binnur Bagci,Sonia Youhanna,Teodor Aastrup,Volker M Lauschke,Cristiano Salata,Nazif Elaldi,Friedemann Weber,Nuria Monserrat,David W Hawman,Heinz Feldmann,Moritz Horn,Josef M Penninger,Ali MirazimiPubMed 38548922
Key facts
- Cell type
- Hep G2
- Species or organism
- Human
- Tissue
- Liver
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
Alternative names
AD2, APOEA, APOE_HUMAN, Apolipoprotein E, Apolipoprotein E3, Apoprotein, LDLCQ5, LPG
Recommended products
APOE KO cell line available to order. Free of charge wild type control provided.
Key facts
- Cell type
- Hep G2
- Species or organism
- Human
- Tissue
- Liver
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Disease
- Hepatocellular Carcinoma
- Concentration
Properties
- Gene name
- APOE
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- MEM + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
- Biological function summary
Four words that are not the target name include ApoE's role as a constituent of chylomicrons VLDL and HDL particles. ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.
- Pathways
Four words that are not the target name include the involvement of ApoE in lipid metabolism and cardiovascular disease pathways. In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.
- Associated diseases and disorders
Four words that are not the target name include ApoE's association with Alzheimer’s disease and cardiovascular diseases. In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Human APOE knockout Hep G2 cell line (ab280875)
False colour image of Western blot: Anti-Apolipoprotein E antibody [EP1374Y] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot Anti-Apolipoprotein E antibody [EP1374Y] ab52607 was shown to bind specifically to Apolipoprotein E. A band was observed at 34-37 kDa in wild-type HepG2 cell lysates with no signal observed at this size in APOE knockout cell line. To generate this image wild-type and APOE knockout HepG2 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Apolipoprotein E antibody [EP1374Y] (Anti-Apolipoprotein E antibody [EP1374Y] ab52607) at 1/1000 dilution
Lane 1: Wild-type HepG2 cell lysate at 20 µg
Lane 2: APOE knockout HepG2 cell lysate at 20 µg
Lane 2: Western blot - Human APOE knockout Hep G2 cell line (ab280875)
Lane 3: Human Liver cell lysate at 20 µg
Lane 4: Human Kidney cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 34 kDa, 34-37 kDa
Western blot - Human APOE knockout Hep G2 cell line (ab280875)
False colour image of Western blot: Anti-Apolipoprotein E antibody [EPR19392] staining at 1/2000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot Anti-Apolipoprotein E antibody [EPR19392] ab183597 was shown to bind specifically to Apolipoprotein E. A band was observed at 34 kDa in wild-type HepG2 cell lysates with no signal observed at this size in APOE knockout cell line. To generate this image wild-type and APOE knockout HepG2 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Apolipoprotein E antibody [EPR19392] (Anti-Apolipoprotein E antibody [EPR19392] ab183597) at 1/2000 dilution
Lane 1: Wild-type HepG2 cell lysate at 20 µg
Lane 2: APOE knockout HepG2 cell lysate at 20 µg
Lane 2: Western blot - Human APOE knockout Hep G2 cell line (ab280875)
Lane 3: Human Liver cell lysate at 20 µg
Lane 4: Human Kidney cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 34 kDa
Sanger Sequencing - Human APOE knockout Hep G2 cell line (ab280875)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com