ASAP1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2
Alternative names
130 kDa phosphatidylinositol 4, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein, 5-biphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, AMAP 1, ANK repeat and PH domain-containing protein 1, ARF GTPase-activating protein 1, ASAP1_HUMAN, Arf-GAP with SH3 domain, CentB4, Centaurin beta 4, DDEF 1, DDEF1 protein, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, KIAA1249, PAG 2, PIP2-dependent ARF1 GAP, ZG 14P
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ASAP1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- ASAP1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASAP1 also known as DDEF1 stands for ADP-Ribosylation Factor GTPase-Activating Protein 1 and has a molecular weight of approximately 130 kDa. It plays a mechanical role in actin cytoskeleton remodeling by regulating ARF (ADP-ribosylation factor) GTPases. These are involved in intracellular vesicular transport. ASAP1 localizes to the cytoplasm and shows high expression in invasive cancer cell lines with noticeable expression noted in tissues such as the brain liver and lung.
- Biological function summary
ASAP1 interacts with components of the focal adhesion complex a group of proteins critical in signaling pathways that connect the extracellular matrix and the actin cytoskeleton. It modulates cell adhesion migration and invasion. ASAP1 binds to proteins like paxillin which further influences the dynamics of actin and membrane trafficking important for cellular rearrangements.
- Pathways
ASAP1 plays roles in actin cytoskeleton reorganization pathways and integrin-mediated signaling pathways. These pathways are essential for cell motility and shape. In these contexts ASAP1 interacts with cortactin an actin-binding protein involved in the regulation of cytoskeletal dynamics necessary for the migration and invasion of cancer cells.
- Associated diseases and disorders
ASAP1 has associations with cancer progression particularly in breast and colorectal cancer. Research indicates that elevated ASAP1 expression correlates with aggressive tumor behavior and poor prognosis. Its interaction with paxillin during disease conditions highlights its involvement in tumor invasiveness and metastasis highlighting its potential as a therapeutic target in oncology.
Product promise
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2 product images
Sanger Sequencing - Human ASAP1 (DDEF1) knockout HeLa cell line (ab265796)
Allele-2: 1 bp insertion in exon 2.
Sanger Sequencing - Human ASAP1 (DDEF1) knockout HeLa cell line (ab265796)
Allele-1: 1 bp deletion in exon 2.
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