Human ATG2B knockout HeLa cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human ATG2B knockout HeLa cell line (AB266010)
Allele-2 : 10 bp deletion in exon 8.
- Sanger seq
Unknown
Sanger Sequencing - Human ATG2B knockout HeLa cell line (AB266010)
Allele-1 : 26 bp deletion in exon 8.
Complementary Products
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein facilitates the transfer of lipids necessary for the elongation of the pre-autophagosomal structure. ATG2B acts as a critical component of the ATG protein complex interacting closely with ATG9A and WIPI1 proteins. Through this complex ATG2B supports the formation and growth of autophagic membranes playing a pivotal role in cellular stress responses. The protein also affects cargo recognition during autophagy thereby influencing the selectivity of molecules degraded within the lysosome.
Pathways
The autophagy process significantly involves ATG2B within its regulatory mechanisms. This protein interacts actively in the mechanistic target of rapamycin (mTOR) signaling pathway which controls autophagy initiation and progression based on cellular nutrient status. Furthermore ATG2B shares collaborations with other related proteins within the phosphatidylinositol 3-kinase (PI3K) pathway linking it to lipid signaling mediations. These associations reinforce its role in maintaining cell survival pathways by degradation of damaged organelles and proteins.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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