Human ATM heterozygous knockout MCF7 cell line

• WB

Lab

Western blot - Human ATM heterozygous knockout MCF7 cell line (AB282630)

Anti-ATM antibody [EPR20100] (ab201022) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab201022 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type MCF7 cell lysates with a reduction in signal observed at this size in ATM heterozygous knockout cell line ab282630. To generate this image, wild-type and ATM heterozygous knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

All lanes:

Western blot - Anti-ATM antibody [EPR20100] - ChIP Grade (<a href='/en-us/products/primary-antibodies/atm-antibody-epr20100-chip-grade-ab201022'>ab201022</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

ATM knockout MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human ATM heterozygous knockout MCF7 cell line (ab282630)

Lane 3:

Wild-type A549 cell lysate at 20 µg

Lane 4:

ATM knockout A549 ab283811 cell lysate at 20 µg

Predicted band size: 351 kDa

Observed band size: 350 kDa

false

• WB

Supplier Data

Western blot - Human ATM heterozygous knockout MCF7 cell line (AB282630)

Western blot : Anti-ATM antibody [Y170] (ab32420) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32420 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type MCF7 cell lysates with a reduction in signal observed at this size in ATM heterozygous knockout cell line ab282630. To generate this image, wild-type and ATM heterozygous knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-ATM antibody [Y170] (<a href='/en-us/products/primary-antibodies/atm-antibody-y170-ab32420'>ab32420</a>) at 1/1000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

ATM knockout MCF7 cell lysate at 20 µg

Lane 3:

Wild-type A549 cell lysate at 20 µg

Lane 4:

ATM knockout A549 ab283811 cell lysate at 20 µg

Observed band size: 350 kDa

false

• Sanger seq

Lab

Sanger Sequencing - Human ATM heterozygous knockout MCF7 cell line (AB282630)

Clone E6 Heterozygote KO, 1bp insertion in Exon 2 and 3.