Human ATM knockout A549 cell line
- Advanced Validation
- What is this?
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(1 Publication)
Human ATM knockout A549 cell line available to order. Recommended control: Human wild-type A549 cell line (ab275463).
View Alternative Names
A-T mutated, A-T mutated homolog, AT1, ATC, ATD, ATDC, ATE, ATM serine/threonine kinase, ATM_HUMAN, Ataxia telangiectasia mutated, Ataxia telangiectasia mutated gene, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated homolog (human), DKFZp781A0353, MGC74674, OTTHUMP00000232981, Serine-protein kinase ATM, Serine/threonine-protein kinase ATM, TEL1, TEL1, telomere maintenance 1, homolog, TELO1, Tefu, Telomere fusion protein
- WB
Lab
Western blot - Human ATM knockout A549 cell line (AB276095)
False colour image of Western blot : Anti-ATM antibody [EPR17059] staining at 1/2000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab199726 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type A549 cell lysates with no signal observed at this size in ATM knockout cell line ab276095 (knockout cell lysate ab283834). To generate this image wild-type and ATM knockout A549 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATM antibody [EPR17059] (<a href='/en-us/products/primary-antibodies/atm-antibody-epr17059-ab199726'>ab199726</a>) at 1/2000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATM knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATM knockout A549 cell line (ab276095)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Predicted band size: 351 kDa
Observed band size: 350 kDa
false
- WB
Lab
Western blot - Human ATM knockout A549 cell line (AB276095)
False colour image of Western blot : Anti-ATM antibody [EPR20100] - ChIP Grade staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab201022 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type A549 cell lysates with no signal observed at this size in ATM knockout cell line ab276095 (knockout cell lysate ab283834). To generate this image wild-type and ATM knockout A549 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATM antibody [EPR20100] - ChIP Grade (<a href='/en-us/products/primary-antibodies/atm-antibody-epr20100-chip-grade-ab201022'>ab201022</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATM knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATM knockout A549 cell line (ab276095)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Predicted band size: 351 kDa
Observed band size: 350 kDa
false
- WB
Lab
Western blot - Human ATM knockout A549 cell line (AB276095)
False colour image of Western blot : Anti-ATM antibody [Y170] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab32420 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type A549 cell lysates with no signal observed at this size in ATM knockout cell line ab276095 (knockout cell lysate ab283834). To generate this image wild-type and ATM knockout A549 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATM antibody [Y170] (<a href='/en-us/products/primary-antibodies/atm-antibody-y170-ab32420'>ab32420</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATM knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATM knockout A549 cell line (ab276095)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Predicted band size: 351 kDa
Observed band size: 350 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human ATM knockout A549 cell line (AB276095)
Knockout achieved by using CRISPR/Cas9, Homozygous : 13bp deletion and 2bp insertion in exon 3
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Cancer research communications 3:1731-1742 PubMed37663435
2023
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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