Human AZIN1 (Antizyme inhibitor 1) knockout HeLa cell line
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AZIN1 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 8 bp deletion in exon 4.
View Alternative Names
AZI, AZIN1_HUMAN, Antizyme inhibitor 1, OAZI, OAZIN, ODC1L, Ornithine decarboxylase antizyme inhibitor
- Sanger seq
Unknown
Sanger Sequencing - Human AZIN1 (Antizyme inhibitor 1) knockout HeLa cell line (AB265296)
Homozygous : 8 bp deletion in exon 4.
- Cell Culture
Unknown
Cell Culture - Human AZIN1 (Antizyme inhibitor 1) knockout HeLa cell line (AB265296)
Representative images of AZIN1 knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AZIN1 significantly influences polyamine biosynthesis by preventing the formation of inhibitory antizyme-ODC complexes. This effectively regulates polyamine levels that are important for cell growth and proliferation. AZIN1 also interacts with other proteins such as ODC forming a regulatory system to balance polyamine homeostasis. The dynamic interaction within this system is critical for cell function affecting several metabolic processes and cellular activities.
Pathways
AZIN1 integrates importantly into the polyamine biosynthesis pathway. This pathway is essential for providing necessary polyamines like putrescine spermidine and spermine central for cellular replication and differentiation. AZIN1's activity can indirectly influence related signaling pathways through polyamine availability impacting proteins like c-Myc which is known to play roles in cell cycle regulation. The control of polyamine metabolism by AZIN1 reflects its broader role in cellular signaling networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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