Human BRPF1 (Peregrin) knockout HEK-293T cell line
- Advanced Validation
- What is this?
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BRPF1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
BR140, BRPF1_HUMAN, Bromodomain and PHD finger containing 1, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140, bromodomain-containing protein, 140kD
- WB
Lab
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (AB266549)
False colour image of Western blot : Anti-Peregrin/BRPF1 antibody - N-terminal staining at 1/500 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab226909 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 160 145 and 138 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Observed bands - 160 145 and 138 kDa
All lanes:
Western blot - Anti-Peregrin/BRPF1 antibody - N-terminal (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-n-terminal-ab226909'>ab226909</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
BRPF1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-brpf1-peregrin-knockout-hek-293t-cell-lysate-ab258795'>ab258795</a>)
Lane 2:
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (ab266549)
Lane 3:
PC-3 cell lysate at 20 µg
Lane 4:
HepG2 cell lysate at 20 µg
Predicted band size: 137 kDa
false
- WB
Lab
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (AB266549)
False colour image of Western blot : Anti-Peregrin/BRPF1 antibody [EPR24069-57] staining at 1/500 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab259840 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 120 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Lanes 1, 2, 3, 5 and 6:
Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-epr24069-57-ab259840'>ab259840</a>) at 1/500 dilution
Lane 4:
Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-epr24069-57-ab259840'>ab259840</a>)
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lanes 2 - 3:
BRPF1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (ab266549)
Lane 4:
Empty
Lane 5:
PC-3 cell lysate at 20 µg
Lane 6:
HepG2 cell lysate at 20 µg
Predicted band size: 137 kDa
Observed band size: 120 kDa
false
- Cell Culture
Lab
Cell Culture - Human BRPF1 (Peregrin) knockout HEK-293T cell line (AB266549)
Representative images BRPF1 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.
- Sanger seq
Unknown
Sanger Sequencing - Human BRPF1 (Peregrin) knockout HEK-293T cell line (AB266549)
Homozygous : 34 bp deletion in exon 2
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Peregrin/BRPF1 is a part of the MOZ/MORF complex which is important for acetylating histone H3 at lysine 23 influencing transcriptional activation. This action aids in transcription regulation and chromatin remodeling facilitating access of transcription factors to DNA. By participating in these processes Peregrin/BRPF1 helps maintain proper gene expression patterns essential for normal cell function and development.
Pathways
BRPF1 has roles in processes like the cell cycle and embryonic development pathways because its histone acetylation influences gene expression. It interacts with other acetyltransferases such as EP300 and CREBBP which play key roles in transcription regulation and chromatin remodeling within these pathways. These interactions highlight BRPF1's critical involvement in coordinating cellular events and developmental processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Cell Lines & Lysates
AB266828
Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Primary Antibodies
AB259840
Anti-Peregrin/BRPF1 antibody [EPR24069-57]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840)](https://content.abcam.com/products/images/peregrin-brpf1-antibody-epr24069-57-ab259840--immunocytochemistry-immunofluorescence-img164599.jpg)
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- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com