SHFL KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3
Alternative names
C19orf66, CS066_HUMAN, Chromosome 19 open reading frame 66, UPF0515 protein C19orf66
Recommended products
SHFL KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- SHFL
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C19orf66 also known as Shiftless or C6orf166 is a protein with a molecular mass of approximately 27 kDa. It functions as an inhibitor of viral RNA replication. Research shows that C19orf66 disrupts viral protein synthesis by interacting with viral factors and initiating their degradation making it important for antiviral defense. This protein is expressed in various tissues with high levels detected in immune-related cells suggesting its role in innate immune response.
- Biological function summary
C19orf66 plays an essential role in modulating immune response against viral pathogens. Its action involves the inhibition of viral replication which helps in controlling the spread of the infection within the host. C19orf66 does not typically form part of large protein complexes but it exerts its effect through direct interaction with viral components preventing their proper assembly and function.
- Pathways
C19orf66 is involved in important antiviral defense mechanisms including those that overlap with the interferon signaling pathway. This protein interacts with interferon-stimulated genes enhancing the host's capability to limit viral infections. Although not directly part of an extensive pathway like NF-kB or MAPK C19orf66 contributes to antiviral defense through its relationship with proteins that are engaged by these pathways during immune responses.
- Associated diseases and disorders
C19orf66 shows implications in viral infections and may play a role in diseases where the immune response is compromised such as chronic viral infections. It has a functional connection with viral proteins which aids in limiting the replication and persistence of viruses. Studies explore its potential usefulness as a therapeutic target especially considering its ability to inhibit the replication of various viruses without directly impacting host cellular machinery.
Product promise
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2 product images
Sanger Sequencing - Human C19orf66 knockout A549 cell line (ab267111)
Allele-2: 2 bp insertion in exon 3.
Sanger Sequencing - Human C19orf66 knockout A549 cell line (ab267111)
Allele-1: 1 bp insertion in exon3
Downloads
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