Human CD167a/DDR1 knockout MCF7 cell line
- Advanced Validation
- What is this?
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- WB
Lab
Western blot - Human CD167a/DDR1 knockout MCF7 cell line (AB280048)
Western blot : Anti-DDR1 antibody [EPR24783-7] (ab288675) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab288675 was shown to bind specifically to DDR1. A band was observed at 100-125 kDa in wild-type HeLa cell lysates with no signal observed at this size in DDR1 knockout cell line ab280048 (knockout cell lysate ab280107). To generate this image, wild-type and DDR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-CD167a/DDR1 antibody [EPR24783-7] (<a href='/en-us/products/primary-antibodies/cd167a-ddr1-antibody-epr24783-7-ab288675'>ab288675</a>) at 1/1000 dilution
Lane 1:
HeLa cell lysate at 20 µg
Lane 2:
NIH/3T3 cell lysate at 20 µg
Lane 3:
T-47D cell lysate at 20 µg
Lane 4:
Human Brain cell lysate at 20 µg
Lane 5:
Human Liver cell lysate at 20 µg
Lane 6:
Mouse Brain cell lysate at 20 µg
Lane 7:
Mouse Liver cell lysate at 20 µg
Lane 8:
Wild-type MCF7 Serum Starved (24 h) Vehicle Control Collagen I (0 ug/mL, 16 h) ab288242 cell lysate at 20 µg
Lane 9:
Wild-type MCF7 Serum Starved (24 h) Treated Collagen I (25 ug/mL, 16 h) ab288241 cell lysate at 20 µg
Lane 10:
DDR1 knockout MCF7 Serum Starved (24 h) Vehicle Control Collagen I (0 ug/mL, 16 h) ab288240 cell lysate at 20 µg
Lane 11:
DDR1 knockout MCF7 Serum Starved (24 h) Treated Collagen I (25 ug/mL, 16 h) ab288238 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 100 kDa,125 kDa
false
- WB
Lab
Western blot - Human CD167a/DDR1 knockout MCF7 cell line (AB280048)
False colour image of Western blot : Anti-DDR1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to DDR1. A band was observed at 105 kDa in treated wild-type MCF7 cell lysates with no signal observed at this size in DDR1 knockout cell line ab280048 (knockout cell lysate ab280107). To generate this image, wild-type and DDR1 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Lane 1:
Human wild-type MCF7 cell lysate - Serum starved collagen I treated (ab288241) at 20 µg
Lane 2:
Human DDR1 (MCK10/NEP) knockout MCF7 cell lysate - Serum starved and collagen I treated (ab288238) at 20 µg
Lane 2:
Western blot - Human CD167a/DDR1 knockout MCF7 cell line (ab280048)
Lane 3:
Human wild-type MCF7 cell lysate - Serum starved control for collagen I (ab288242) at 20 µg
Lane 4:
Human DDR1 (MCK10/NEP) knockout MCF7 cell lysate -Serum starved and control for collagen I (ab288240) at 20 µg
Lane 5:
A549 cell lysate at 20 µg
Lane 6:
HeLa cell lysate at 20 µg
false
- Sanger seq
Lab
Sanger Sequencing - Human CD167a/DDR1 knockout MCF7 cell line (AB280048)
59 bp Deletion in Exon 5
Complementary Products
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
MEM + 10% FBS + 0.01 mg/ml bovine insulin
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.
Pathways
CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com