Human CD1C knockout Jurkat cell line
- Advanced Validation
- What is this?
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CD1C KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 100%.
View Alternative Names
BDCA1, CD1, CD1C antigen, CD1C antigen c polypeptide, CD1C_HUMAN, CD1c molecule, Cortical thymocyte antigen CD1C, Differentiation antigen CD1 alpha 3, R7, T-cell surface glycoprotein CD1c
- Flow Cyt
Lab
Flow Cytometry - Human CD1C knockout Jurkat cell line (AB273867)
Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout (red line) cells (ab273867) stained with ab190305. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab190305) (1x106 in 100 μl at 0.2 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG1, kappa (ab170190) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
- Flow Cyt
Unknown
Flow Cytometry - Human CD1C knockout Jurkat cell line (AB273867)
Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout Jurkat cells (ab273867) stained with ab273049 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab273049) (1x106 in 100 μl at 0.2 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
- NGS
Supplier Data
Next Generation Sequencing - Human CD1C knockout Jurkat cell line (AB273867)
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift : 100%
- NGS
Supplier Data
Next Generation Sequencing - Human CD1C knockout Jurkat cell line (AB273867)
1 bp deletion after Phe111 of the WT protein
Reactivity data
Product details
Recommended control: Human wild-type Jurkat cell line (ab271143). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of CD1c involves the presentation of lipid antigens which plays a role in immune recognition. CD1c is a part of a complex that includes β2-microglobulin which stabilizes its structure for antigen presentation. CD1c contributes to the immune surveillance by enabling the recognition of microbial lipid antigens as well as self-lipids by specific T cell subsets. This function supports the body's immune defense by identifying and reacting to potential pathogenic lipids.
Pathways
CD1c is involved in immune response pathways that center around antigen presentation. It interacts with other proteins like CD1a CD1b and CD1d expanding the range of antigens that T cells can recognize. CD1c participates in the lipid antigen presentation pathway influencing both innate and adaptive immunity. The presentation of lipid antigens by CD1c to T cells can trigger signaling pathways that affect both immune activation and resolution.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB270797
Anti-CD1c antibody [EPR23189-196] - BSA and Azide free
BOND RX™ Validated|RabMAb|Recombinant
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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