Human CD791 knockout Raji cell line
- Advanced Validation
- What is this?
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CD79A KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
B lymphocyte-specific MB1 protein, B-cell antigen receptor complex-associated protein alpha chain, CD79A antigen, CD79A_HUMAN, CD79a antigen (immunoglobulin-associated alpha), CD79a molecule, immunoglobulin-associated alpha, Ig-alpha, IgM-alpha, Immunoglobulin-associated alpha, Ly54, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein
- WB
Lab
Western blot - Human CD791 knockout Raji cell line (AB274911)
All lanes:
Western blot - Anti-CD79a antibody [EPR3619] (<a href='/en-us/products/primary-antibodies/cd79a-antibody-epr3619-ab133483'>ab133483</a>) at 1/1000 dilution
Lane 1:
Wild-type Raji cell lysate at 20 µg
Lane 2:
CD79A knockout Raji cell lysate at 20 µg
Lane 3:
Daudi cell lysate at 20 µg
Secondary
Lanes 1 - 3:
Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 3:
Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 50 kDa
false
- WB
Lab
Western blot - Human CD791 knockout Raji cell line (AB274911)
False colour image of Western blot : Anti-CD79a antibody [EP3618] staining at 1/5000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab79414 was shown to bind specifically to CD79a. A band was observed at 40-50 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79A knockout cell line ab274911 (knockout cell lysate ab281361). To generate this image wild-type and CD79A knockout Raji cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-CD79a antibody [EP3618] (<a href='/en-us/products/primary-antibodies/cd79a-antibody-ep3618-ab79414'>ab79414</a>) at 1/5000 dilution
Lane 1:
Wild-type Raji cell lysate at 20 µg
Lane 2:
CD79A knockout Raji cell lysate at 20 µg
Lane 2:
Western blot - Human CD791 knockout Raji cell line (ab274911)
Lane 3:
Daudi cell lysate at 20 µg
Lane 4:
HAP1 cell lysate at 20 µg
Predicted band size: 25 kDa
Observed band size: 40-50 kDa
false
- NGS
Lab
Next Generation Sequencing - Human CD791 knockout Raji cell line (AB274911)
14 bp deletion after Ser58 of the WT protein
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x105 - 5x105 cells/mL(for initial passages it is recomended to culture the cells in the higher range of recomended seeding density). Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 4x105 cells/mL is recommended.
- A maximum of 3x106 viable cells/mL is obtainable.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein acts as an essential mediator in the immune system. CD79a together with CD79b forms part of the BCR complex critical for B cell development and maturation. The BCR complex activates signaling cascades that influence B cell fate decisions such as proliferation differentiation and apoptosis. By transmitting signals from the BCR CD79a helps regulate these cellular functions necessary for efficient immune defense.
Pathways
CD79a plays a pivotal role in the B cell receptor signaling pathway. This pathway involves signaling molecules such as spleen tyrosine kinase (SYK) and phosphoinositide 3-kinase (PI3K) which are activated upon BCR engagement. CD79a acts in concert with other proteins including CD79b and the IgM receptor facilitating downstream signaling cascades that ensure proper B cell responses. Its role is important for linking extracellular antigen recognition to intracellular signaling events.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Suspension
Gender
Male
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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