CDC14B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and 2 bp insertion in exon 3 and 8 bp deletion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and 2 bp insertion in exon 3 and 8 bp deletion in exon 3
Alternative names
CC14B_HUMAN, CDC 14B3, CDC14 cell division cycle 14 homolog B, CDC14 homolog B, Cdc 14B, Cdc 14B1, Cdc 14B2, Cell division cycle 14 homolog B, Dual specificity protein phosphatase CDC14B, OTTHUMP00000063774, hCDC 14B
Recommended products
CDC14B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and 2 bp insertion in exon 3 and 8 bp deletion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and 2 bp insertion in exon 3 and 8 bp deletion in exon 3
- Concentration
Properties
- Gene name
- CDC14B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdc14B also known as Cell Division Cycle 14B is a serine/threonine-protein phosphatase with a molecular mass of approximately 62 kDa. This protein localizes mainly to the nucleolus but can also be found in the cytoplasm during the cell cycle. It plays a significant role in dephosphorylating substrates involved in cell cycle regulation. Cdc14B is widely expressed across various human tissues including high expression levels in the testes where it serves essential regulatory functions.
- Biological function summary
Cdc14B regulates exit from mitosis and cytokinesis. It functions as part of a regulatory network that controls the transition from mitosis to interphase. In this process Cdc14B dephosphorylates key proteins involved in spindle disassembly and chromosome segregation ensuring proper cell cycle progression. While it does not form a complex on its own Cdc14B interacts with other regulatory proteins to maintain cellular homeostasis and genomic stability.
- Pathways
Cdc14B plays a critical role in the DNA damage response and the mitotic exit network. It interacts with proteins such as Cdh1 and Securin to mediate cell cycle checkpoints and ensure proper timing of mitotic exit. Cdc14B's ability to dephosphorylate specific substrates allows it to regulate these pathways effectively placing it centrally within the regulatory framework that safeguards cell cycle accuracy.
- Associated diseases and disorders
Alterations in Cdc14B expression or function have been linked to cancer development due to its role in cell cycle regulation and genomic stability. Aberrant Cdc14B activity can contribute to unchecked cell division a hallmark of many cancers. Additionally its dysfunction associates with neurodegenerative diseases where improper cell cycle re-entry of neurons leads to cell death. In cancer Cdc14B's interaction with proteins like p53 and Mdm2 highlights its importance in tumor suppression pathways.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (ab266580)
Allele-3: 2 bp insertion in exon 3.
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (ab266580)
Allele-1: 8 bp deletion in exon3
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (ab266580)
Allele-2: 2 bp deletion in exon 3.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com