Human CDC14B knockout HEK-293T cell line
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CDC14B KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3 and 2 bp insertion in exon 3 and 8 bp deletion in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
CC14B_HUMAN, CDC 14B3, CDC14 cell division cycle 14 homolog B, CDC14 homolog B, Cdc 14B, Cdc 14B1, Cdc 14B2, Cell division cycle 14 homolog B, Dual specificity protein phosphatase CDC14B, OTTHUMP00000063774, hCDC 14B
- Sanger seq
Unknown
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (AB266580)
Allele-3 : 2 bp insertion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (AB266580)
Allele-2 : 2 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human CDC14B knockout HEK-293T cell line (AB266580)
Allele-1 : 8 bp deletion in exon3
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cdc14B regulates exit from mitosis and cytokinesis. It functions as part of a regulatory network that controls the transition from mitosis to interphase. In this process Cdc14B dephosphorylates key proteins involved in spindle disassembly and chromosome segregation ensuring proper cell cycle progression. While it does not form a complex on its own Cdc14B interacts with other regulatory proteins to maintain cellular homeostasis and genomic stability.
Pathways
Cdc14B plays a critical role in the DNA damage response and the mitotic exit network. It interacts with proteins such as Cdh1 and Securin to mediate cell cycle checkpoints and ensure proper timing of mitotic exit. Cdc14B's ability to dephosphorylate specific substrates allows it to regulate these pathways effectively placing it centrally within the regulatory framework that safeguards cell cycle accuracy.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com