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CEBPA KO cell line available to order. Free of charge wild type control provided.

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Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Form

Liquid

Alternative names

Recommended products

CEBPA KO cell line available to order. Free of charge wild type control provided.

Key facts

Cell type

A549

Form

Liquid

Disease

Carcinoma

Concentration
Loading...

Properties

Gene name

CEBPA

Gene editing type

Knockout

Gene editing method

CRISPR technology

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

  • Do not allow the cell density to exceed 7x104 cells/cm2.

Culture medium

F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CEBP Alpha also known as CEBPA is a transcription factor belonging to the CCAAT/enhancer-binding protein family. It is composed of a molecular mass approximately between 42 to 45 kDa and is characterized by the leucine zipper motif which contributes to its function in dimerization and DNA binding. CEBP Alpha is expressed in various tissues with notable expression in the liver adipose tissue and hematopoietic cells where it plays a significant role in regulating gene expression related to growth arrest and differentiation.

Biological function summary

CEBP Alpha regulates important cellular processes by acting as a transcriptional regulator. It participates in the control of cellular differentiation proliferation and metabolism. CEBP Alpha forms homo- or heterodimers with other proteins of the CEBP family to exert its functions. It induces expression of genes involved in differentiation of myeloid progenitors into granulocytes and macrophages. The protein also promotes adipogenesis by activating genes responsible for the development and function of adipocytes.

Pathways

CEBP Alpha participates actively in signaling pathways that involve cell cycle control and hematopoiesis. In the hematopoietic pathway CEBP Alpha influences the differentiation of progenitor cells into mature blood cells. Additionally it interacts with other proteins such as PU.1 an important regulator in the myeloid lineage and through the MAPK pathway influences growth arrest and differentiation responses. These interactions highlight its essential role in maintaining the balance between cell proliferation and differentiation.

Associated diseases and disorders

Alterations in the function or expression of CEBP Alpha have consequential links to acute myeloid leukemia (AML) and obesity. Mutations or disruptions in the CEBP Alpha gene can lead to impaired granulocyte differentiation contributing to the pathogenesis of AML. Additionally CEBP Alpha through its role in adipogenesis is associated with metabolic disorders such as obesity. The protein's interaction with other transcription factors like FLT3 and GATA-2 in the context of these diseases highlights its critical involvement in maintaining normal physiological functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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