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HDHD5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.

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Images

Western blot - Human CECR5 knockout HEK-293T cell line (AB266708), expandable thumbnail
  • Cell Culture - Human CECR5 knockout HEK-293T cell line (AB266708), expandable thumbnail
  • Sanger Sequencing - Human CECR5 knockout HEK-293T cell line (AB266708), expandable thumbnail
  • Sanger Sequencing - Human CECR5 knockout HEK-293T cell line (AB266708), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6

Alternative names

A930002G03Rik, CECR5_HUMAN, Cat eye syndrome chromosome region, candidate 5, Cat eye syndrome chromosome region, candidate 5 homolog, Cat eye syndrome critical region protein 5, MGC25951, OTTMUSP00000025905

Recommended products

HDHD5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 6 and 1 bp deletion in exon 6
Concentration
Loading...

Properties

Gene name
HDHD5
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CECR5 also known as Cat Eye Syndrome Critical Region Candidate 5 encodes a protein with a mass of about 48 kDa. This protein shows expression in multiple tissues such as the brain kidney and liver. Its mechanical role involves binding interactions that help in signaling and cellular communication processes. The protein's structure enables it to participate effectively in these interactions making it a point of interest for further study in these specific tissues.

Biological function summary

CECR5 serves an important function in cellular signaling networks and is not typically found as part of a larger complex. The protein plays a role in modulating certain cellular responses possibly impacting processes like differentiation and growth. Researchers have identified CECR5 involvement in cellular mechanisms that govern normal development and maintenance hinting at its broader influence in cellular homeostasis.

Pathways

CECR5 engages in the MAPK signaling pathway which is instrumental in transferring extracellular signals to intracellular responses. The MAPK pathway involves proteins like ERK and JNK with which CECR5 may interact or have regulatory effects. Additionally it influences signaling via the PI3K-AKT pathway a pathway that controls many aspects of cell survival growth and metabolism. This association suggests CECR5's potential roles in important cellular activities and regulatory mechanisms.

Associated diseases and disorders

Alterations in CECR5 expression or function relate to Cat Eye Syndrome and possibly neurodevelopmental disorders. In Cat Eye Syndrome the overexpression or structural changes of CECR5 may disrupt normal development alongside chromosomal duplications involving the region. Connections also appear between CECR5 and other proteins like PAX6 in the context of its role in developmental processes. Understanding these relationships further explains CECR5's impact on these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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