Human CHEK2 (Chk2) knockout A549 cell line
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(1 Publication)
CHEK2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 151 bp deletion in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
CHEK 2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), CHK2_HUMAN, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, HuCds 1, LFS 2, PP1425, Rad53 homolog, Serine/threonine-protein kinase Chk2, hCds1
- WB
Lab
Western blot - Human CHEK2 (Chk2) knockout A549 cell line (AB276098)
Lanes 1 - 4:
Western blot - Anti-Chk2 antibody [EPR19482] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr19482-ab207446'>ab207446</a>) at 1/1000 dilution
Lanes 1 - 4:
Western blot - Anti-Chk2 antibody [EPR19482] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr19482-bsa-and-azide-free-ab251477'>ab251477</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
Western blot - Human CHEK2 (Chk2) knockout A549 cell line (ab276098)
Lane 2:
CHEK2 knockout A549 cell lysate at 20 µg
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 67 kDa
false
- WB
Lab
Western blot - Human CHEK2 (Chk2) knockout A549 cell line (AB276098)
Lanes 1 - 4:
Western blot - Anti-Chk2 antibody [EPR4325] (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr4325-ab109413'>ab109413</a>) at 1/5000 dilution
Lanes 1 - 4:
Western blot - Anti-Chk2 antibody [EPR4325] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/chk2-antibody-epr4325-bsa-and-azide-free-ab227998'>ab227998</a>) at 1/5000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
Western blot - Human CHEK2 (Chk2) knockout A549 cell line (ab276098)
Lane 2:
CHEK2 knockout A549 cell lysate at 20 µg
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
MDA-MB-231 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 67 kDa
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human CHEK2 (Chk2) knockout A549 cell line (AB276098)
151 bp deletion in exon 2.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 15:10782 PubMed39737931
2024
Applications
Unspecified application
Species
Unspecified reactive species
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Advanced Validation
- 0
- 0
Primary Antibodies
AB109413
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk2 antibody [EPR4325] (AB109413)](https://content.abcam.com/products/images/chk2-antibody-epr4325-ab109413--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91668.jpg)
- 5
- 9
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com