Human CLTA (Clathrin light chain A) knockout HEK-293T cell line
- Advanced Validation
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
CLTA KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
Lca, CLTA, Clathrin light chain A
- WB
Lab
Western blot - Human CLTA (Clathrin light chain A) knockout HEK-293T cell line (AB267334)
False colour image of Western blot : Anti-Clathrin light chain antibody [EPR24231-72] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab271185 was shown to bind specifically to Clathrin light chain. A band was observed at 35 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in CLTA knockout cell line ab267334 (knockout cell lysate ab258366). To generate this image wild-type and CLTA knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-Clathrin light chain antibody [EPR24231-72] (<a href='/en-us/products/primary-antibodies/clathrin-light-chain-antibody-epr24231-72-ab271185'>ab271185</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
CLTA knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human CLTA (Clathrin light chain A) knockout HEK-293T cell line (ab267334)
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
HeLa cell lysate at 20 µg
Predicted band size: 27 kDa
Observed band size: 35 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell line (AB267334)
Allele-2 : 1 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human CLTA (Clathrin light chain A) knockout HEK-293T cell line (AB267334)
Allele-1 : 2 bp deletion in exon1
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALK-1 antibody [MM0015-8G33] (AB51870)](https://content.abcam.com/products/images/alk-1-antibody-mm0015-8g33-ab51870--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121358.jpg)
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Clathrin light chain modulates the lattice dynamics by interacting with clathrin heavy chains in a clathrin protein complex. This interaction supports the stability and assembly of the clathrin lattice affecting the efficiency and specificity of endocytic vesicle formation. Clathrin light chain acts in concert with other accessory proteins to regulate clathrin coat assembly and disassembly playing a dynamic role in cellular trafficking processes.
Pathways
The clathrin light chain participates in essential cellular pathways such as the clathrin-mediated endocytosis and synaptic vesicle recycling pathways. These pathways facilitate the internalization and recycling of membrane proteins and lipids ensuring cellular homeostasis. In these processes the clathrin light chain works closely with related proteins such as dynamin and adaptin to control vesicle scission and cargo selection.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com