COL4A6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 24 and 2 bp deletion in exon 24 and 4 bp deletion in exon 24.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 24 and 2 bp deletion in exon 24 and 4 bp deletion in exon 24
Alternative names
COL4A6, Collagen Alpha 6(IV), Collagen IV alpha 6 polypeptide, Collagen alpha 6(IV) chain, Collagen of basement membrane alpha 6, Collagen type IV alpha 6, MGC88184, Type IV alpha 6 collagen, dJ889N15.4
Recommended products
COL4A6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 24 and 2 bp deletion in exon 24 and 4 bp deletion in exon 24.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 24 and 2 bp deletion in exon 24 and 4 bp deletion in exon 24
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- COL4A6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen IV alpha 6 also known as COL4A6 forms a part of the collagen IV family. This protein acts as a structural component in the basement membrane playing a role in maintaining the integrity and stability of these membranes. Weighing about 160 kDa COL4A6 is expressed in a variety of tissues such as the kidney lung and testis. The local expression in these tissues suggests its key role in supporting organ-specific structures.
- Biological function summary
As part of the collagen IV network COL4A6 contributes to the formation of the heterotrimeric type IV collagen molecules. These trimers are composed of three alpha chains that associate to form a complex structure necessary for providing mechanical support and filtration functions in the basement membrane. The involvement of COL4A6 in these processes highlights its important role in tissue morphogenesis and maintenance.
- Pathways
The function of COL4A6 integrates into major signaling cascades like the integrin-mediated signaling pathway and the extracellular matrix (ECM) remodeling pathway. Within these pathways COL4A6 collaborates with other collagen IV proteins such as COL4A1 and COL4A5 to interact with integrins. These interactions are critical for signal transduction processes that regulate cell adhesion migration and differentiation thereby maintaining tissue architecture.
- Associated diseases and disorders
Mutations or alterations in COL4A6 are linked to Alport syndrome and some forms of nephropathy. In Alport syndrome there is a direct connection with COL4A5 another collagen IV family member which often coexists with COL4A6 in forming critical basement membrane structures. Dysfunction in these proteins leads to compromised membrane stability and filtration resulting in the characteristic symptoms of kidney disease and hearing loss seen in affected individuals.
Product promise
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3 product images
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell line (ab265914)
Allele-1: 4 bp deletion in exon 24.
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell line (ab265914)
Allele-3: 1 bp insertion in exon 24.
Sanger Sequencing - Human COL4A6 (Collagen IV alpha 6) knockout HeLa cell line (ab265914)
Allele-2: 2 bp deletion in exon 24.
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