Human COLGALT1 (GLT25D1) knockout HEK-293T cell line
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COLGALT1 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
View Alternative Names
FLJ22329, Glycosyltransferase 25 domain containing 1, Hydroxylysine galactosyltransferase 1, Procollagen galactosyltransferase 1
- Sanger seq
Unknown
Sanger Sequencing - Human COLGALT1 (GLT25D1) knockout HEK-293T cell line (AB266750)
Homozygous : 5 bp deletion in exon2
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GLT25D1 plays a significant role in the post-translational modification of collagen. It participates in forming the galactosylhydroxylysine residues which are important for collagen's structural integrity and function. The enzyme may function as part of a multiprotein complex which ensures efficient modification of the collagen fibers during their biosynthesis. Through these modifications the enzyme affects the fibrillar assembly that is essential for connective tissue strength and elasticity.
Pathways
The role of GLT25D1 is prominent in the collagen synthesis pathway. This pathway involves not only the synthesis of collagen polypeptides but also their modification folding and secretion. The GLT25D1 enzyme works in conjunction with other glycosyltransferases like galactosyltransferase 2 (GLT25D2) to ensure collagen molecules are properly glycosylated and transported through the secretory pathway. These enzymes together regulate the mechanical properties of the extracellular matrix which is important for tissue function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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