CPSF7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3
Alternative names
CFIm59, CPSF 59 kDa subunit, CPSF7_HUMAN, Cleavage and polyadenylation specificity factor 59 kDa subunit, Cleavage and polyadenylation specificity factor 7, Cleavage and polyadenylation specificity factor subunit 7, FLJ12529 pre mRNA cleavage factor I 59 kDa subunit, FLJ39024, MGC9315, Pre-mRNA cleavage factor Im 59 kDa subunit
Recommended products
CPSF7 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3
- Concentration
Properties
- Gene name
- CPSF7
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPSF7 also known as cleavage and polyadenylation specificity factor subunit 7 is a protein that participates in mRNA processing. With a mass of approximately 55 kDa CPSF7 resides in the nucleus of cells where it plays a role in the cleavage and polyadenylation of pre-mRNA. This protein is expressed in various tissues emphasizing its broad role in gene expression regulation across different cell types.
- Biological function summary
CPSF7 functions in the formation of the cleavage and polyadenylation complex which is essential for proper mRNA maturation. It interacts with other subunits in this complex to ensure accurate mRNA 3' end processing. This protein participates in determining the polyadenylation site a critical step that influences mRNA stability export and translation.
- Pathways
CPSF7 integrates into the post-transcriptional regulation of gene expression. It plays important roles within the mRNA processing pathway and is linked to the regulation of the cell cycle. CPSF7 interacts with other proteins such as CPSF3 within these pathways to mediate these biological processes that are pivotal for efficient gene expression and cell function.
- Associated diseases and disorders
Aberrations in CPSF7 function relate to cancer and autoimmune diseases. In cancer altered expression of CPSF7 may affect tumor growth and progression potentially through interactions with other proteins like CPSF73. Autoimmune diseases could also involve CPSF7 due to disruptions in mRNA processing which might affect immune cell function and regulation.
Product promise
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1 product image
Sanger Sequencing - Human CPSF7 knockout HEK-293T cell line (ab266275)
Homozygous: Insertion of the selection cassette in exon3
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