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CPT1A KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.

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Images

Western blot - Human CPT1A knockout HEK-293T cell line (AB266319), expandable thumbnail
  • Western blot - Human CPT1A knockout HEK-293T cell line (AB266319), expandable thumbnail
  • Sanger Sequencing - Human CPT1A knockout HEK-293T cell line (AB266319), expandable thumbnail
  • Sanger Sequencing - Human CPT1A knockout HEK-293T cell line (AB266319), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 3 and Insertion of the selection cassette in exon 3

Alternative names

CPT 1, CPT I, CPT1-L, CPT1A_HUMAN, CPTI-L, Carnitine O palmitoyltransferase 1 liver isoform, Carnitine O palmitoyltransferase I liver isoform, Carnitine O-palmitoyltransferase 1, Carnitine O-palmitoyltransferase I, Carnitine palmitoyltransferase 1A, Carnitine palmitoyltransferase 1A (liver), Carnitine palmitoyltransferase I, Carnitine palmitoyltransferase I liver, L CPT1, liver isoform

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CPT1A KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 3 and Insertion of the selection cassette in exon 3
Concentration
Loading...

Properties

Gene name
CPT1A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.

Biological function summary

CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.

Pathways

CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.

Associated diseases and disorders

CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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