Human CRAT knockout HEK-293T cell line
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Human CRAT knockout HEK-293T cell line available to order. Recommended control: Human wild-type HEK293T cell line (ab255449).
View Alternative Names
CACP_HUMAN, Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CrAT
- Sanger seq
Unknown
Sanger Sequencing - Human CRAT knockout HEK-293T cell line (AB266361)
Allele-1 : 5 bp deletion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human CRAT knockout HEK-293T cell line (AB266361)
Allele-2 : 1 bp deletion in exon 6.
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Carnitine O-acetyltransferase facilitates the transportation of acyl groups across the mitochondrial membranes influencing energy homeostasis by converting excess acyl-CoA to acylcarnitine. It does not form part of a larger protein complex but operates as a singular catalytic entity. The enzyme's function in balancing acyl-CoA and free CoA levels is essential for maintaining cellular metabolic flexibility allowing cells to switch energy sources efficiently when necessary.
Pathways
Carnitine O-acetyltransferase sits within the fatty acid oxidation pathway aiding in the linkage between carbohydrate and lipid metabolism. It interacts with proteins like carnitine palmitoyltransferase I (CPT I) and acetyl-CoA carboxylase important for controlling the flow of long-chain fatty acids into mitochondria for β-oxidation. The action of CRAT impacts the ketogenesis pathway assisting in maintaining energy production through conversion of stored fats when glucose is not readily available.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com