CRIM1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp insertion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp insertion in exon 3
Alternative names
AU015004, CRIM1_HUMAN, Cysteine rich motor neuron 1 protein, Cysteine rich transmembrane BMP regulator 1, Cysteine rich transmembrane BMP regulator 1 (chordin like), Cysteine-rich motor neuron protein 1, Cysteine-rich repeat-containing protein S52, MGC138194, Processed cysteine-rich motor neuron 1 protein, S52
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CRIM1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp insertion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp insertion in exon 3
- Concentration
Properties
- Gene name
- CRIM1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRIM1 also known as cysteine-rich motor neuron 1 protein has a molecular mass of around 110 kDa. As a transmembrane protein it localizes mainly to the endoplasmic reticulum. Researchers have detected its expression in various tissues including the retina kidneys brain and spinal cord. It contains a distinctive cysteine-rich motif and plays roles in cellular adhesion and growth factor regulation.
- Biological function summary
The function of CRIM1 extends to the modulation of growth factor activity particularly by interacting with bone morphogenetic proteins (BMPs). This interaction suggests its potential involvement in developmental processes. Studies have indicated that CRIM1 might participate in forming protein complexes related to cell surface receptors influencing cell signaling pathways that govern growth and differentiation.
- Pathways
CRIM1's involvement in BMP signaling and angiogenesis highlights its significance within these biological pathways. BMPs which are growth factors play a part in bone and cartilage development therefore CRIM1's regulatory action can affect these processes. Additionally CRIM1 interacts with proteins like noggin in the BMP pathway to modulate cellular responses to extracellular signals contributing to developmental and morphogenic outcomes.
- Associated diseases and disorders
CRIM1 links to congenital abnormalities and ocular disorders. Its known interaction with BMPs associates it with conditions like kidney dysplasia where the disruption of normal signaling can lead to organ malformations. In the context of eye development CRIM1's interaction with BMPs and noggin is important for retinal and lens development. Understanding CRIM1's role in these pathways could offer insights into therapeutic targets for congenital and degenerative diseases.
Product promise
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Terms & Conditions.
3 product images
Cell Culture - Human CRIM1 knockout HEK-293T cell line (ab267286)
Representative images of CRIM1 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.
Sanger Sequencing - Human CRIM1 knockout HEK-293T cell line (ab267286)
Allele-2: 2 bp insertion in exon 3.
Sanger Sequencing - Human CRIM1 knockout HEK-293T cell line (ab267286)
Allele-1: 1 bp deletion in exon3
Downloads
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