Human CTNNB1 (beta Catenin) knockout Hep G2 cell line
- Advanced Validation
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(0 Publication)
- WB
Lab
Western blot - Human CTNNB1 (beta Catenin) knockout Hep G2 cell line (AB277911)
False colour image of Western blot : Anti-beta Catenin non-phospho S37/T41 antibody [EPR23969-131] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab246504 was shown to bind specifically to beta Catenin non-phospho(active) S37/T41. A band was observed at 90 kDa in wild-type HepG2 cell lysates with no signal observed at this size in CTNNB1 knockout cell line. To generate this image, wild-type and CTNNB1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-beta Catenin non-phospho S37/T41 antibody [EPR23969-131] (<a href='/en-us/products/primary-antibodies/beta-catenin-non-phospho-s37-t41-antibody-epr23969-131-ab246504'>ab246504</a>) at 1/1000 dilution
Lane 1:
Wild-type HepG2 cell lysate at 20 µg
Lane 2:
CTNNB1 knockout HepG2 cell lysate at 20 µg
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
A431 cell lysate at 20 µg
Predicted band size: 85 kDa
Observed band size: 90 kDa
false
- WB
Lab
Western blot - Human CTNNB1 (beta Catenin) knockout Hep G2 cell line (AB277911)
False colour image of Western blot : Anti-beta Catenin antibody [E247] - ChIP Grade staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32572 was shown to bind specifically to beta Catenin. A band was observed at 85 kDa in wild-type HepG2 cell lysates with no signal observed at this size in CTNNB1 knockout cell line (ab277911). To generate this image, wild-type and CTNNB1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-beta Catenin antibody [E247] - ChIP Grade (<a href='/en-us/products/primary-antibodies/beta-catenin-antibody-e247-chip-grade-ab32572'>ab32572</a>) at 1/5000 dilution
Lane 1:
Wild-type HepG2 cell lysate at 20 µg
Lane 2:
CTNNB1 knockout HepG2 cell lysate at 20 µg
Lane 2:
Western blot - Human CTNNB1 (beta Catenin) knockout Hep G2 cell line (ab277911)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
A431 cell lysate at 20 µg
Predicted band size: 85 kDa
Observed band size: 85 kDa
false
- WB
Lab
Western blot - Human CTNNB1 (beta Catenin) knockout Hep G2 cell line (AB277911)
False colour image of Western blot : Anti-beta Catenin antibody [EP690Y] staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab68183 was shown to bind specifically to beta Catenin. A band was observed at 85 kDa in wild-type HepG2 cell lysates with no signal observed at this size in CTNNB1 knockout cell line. To generate this image, wild-type and CTNNB1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-beta Catenin antibody [EP690Y] (<a href='/en-us/products/primary-antibodies/beta-catenin-antibody-ep690y-ab68183'>ab68183</a>) at 1/500 dilution
Lane 1:
Wild-type HepG2 cell lysate at 20 µg
Lane 2:
CTNNB1 knockout HepG2 cell lysate at 20 µg
Lane 2:
Western blot - Human CTNNB1 (beta Catenin) knockout Hep G2 cell line (ab277911)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
A431 cell lysate at 20 µg
Predicted band size: 85 kDa
Observed band size: 85 kDa
false
Complementary Products
Primary Antibodies
AB32572
Anti-beta Catenin antibody [E247] - ChIP Grade
BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)](https://content.abcam.com/products/images/beta-catenin-antibody-e247-chip-grade-ab32572--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93749.jpg)
- 5
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Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
MEM + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin's interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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