Human CTSC (Cathepsin C) knockout HeLa cell line
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- sELISA
Supplier Data
Sandwich ELISA - Human CTSC (Cathepsin C) knockout HeLa cell line (AB265822)
Sandwich ELISA of ab314793 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL. Interpolated concentrations of native Cathepsin C in human control wild type HeLa cell and CTSC (Cathepsin C) knockout HeLa cell based on 2.5 µg/mL extract loads. The concentrations of Cathepsin C were measured in duplicate and interpolated from the Cathepsin C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin C concentration was determined to be 310.1 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255928) and undetectable in CTSC (Cathepsin C) knockout HeLa extract (Human CTSC (Cathepsin C) knockout HeLa cell line ab265822).
- Sanger seq
Unknown
Sanger Sequencing - Human CTSC (Cathepsin C) knockout HeLa cell line (AB265822)
Allele-1 : 32 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human CTSC (Cathepsin C) knockout HeLa cell line (AB265822)
Allele-2 : 19 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human CTSC (Cathepsin C) knockout HeLa cell line (AB265822)
Allele-3 : 17 bp deletion in exon 1.
Complementary Products
Primary Antibodies
AB314644
Anti-Cathepsin C antibody [EPR26636-76]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin C antibody [EPR26636-76] (AB314644)](https://content.abcam.com/products/images/cathepsin-c-antibody-epr26636-76-ab314644--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421860.jpg)
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Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme contributes significantly to the immune system. It forms part of a multi-enzyme complex that processes proenzymes such as granzymes and cathepsin G into their active forms. Through these actions Cathepsin C regulates cellular activities linked to inflammation and apoptosis. The proteolytic activity it provides is critical for the functioning of immune-related cells facilitating defense mechanisms against pathogens.
Pathways
Cathepsin C plays roles in the inflammatory and immune response pathways. It connects to the granzyme and lysosomal pathways important in processing and regulating granzyme activities critical for cytotoxic T lymphocyte functions. Cathepsin C maintains connections with proteins like granzymes and cathepsin G each involved in mediating immune responses and cellular death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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