Human CXCL1 (GRO alpha) knockout HeLa cell line
- Advanced Validation
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CXCL1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 62 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
C-X-C motif chemokine 1, CINC-1, CXCL1, Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), Cytokine-induced neutrophil chemoattractant 1, Fibroblast secretory protein, Fsp, GRO protein, alpha, GRO-alpha(1-73), GRO-alpha(6-73), GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), GROA_HUMAN, Gro, Gro 1, Gro1 oncogene, Growth-regulated alpha protein, KC, KC chemokine, mouse, homolog of, MGSA, MGSA alpha, MGSA-a, Melanoma growth stimulatory activity, Melanoma growth stimulatory activity alpha, N51, NAP-3, Neutrophil-activating protein 3, Platelet-derived growth factor-inducible protein KC, Scyb 1, Secretory protein N51, Small inducible cytokine subfamily B, member 1, chemokine (C-X-C motif) ligand 1
- WB
Lab
Western blot - Human CXCL1 (GRO alpha) knockout HeLa cell line (AB261774)
False colour image of Western blot : Anti-CXCL1/GRO alpha antibody [EPR19892] staining at 1/100 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab206411 was shown to bind specifically to CXCL1/GRO alpha. A band was observed at 8 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CXCL1 knockout cell line ab261774 (knockout cell lysate ab257079). To generate this image, wild-type and CXCL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.
All lanes:
Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (<a href='/en-us/products/primary-antibodies/cxcl1-gro-alpha-antibody-epr19892-ab206411'>ab206411</a>) at 1/100 dilution
Lane 1:
Wild-type HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 2:
CXCL1 knockout HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate at 20 µg
Lane 2:
Western blot - Human CXCL1 (GRO alpha) knockout HeLa cell line (ab261774)
Predicted band size: 11 kDa
Observed band size: 8 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CXCL1 (GRO alpha) knockout HeLa cell line (AB261774)
Allele-1 : 62 bp insertion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human CXCL1 (GRO alpha) knockout HeLa cell line (AB261774)
Allele-2 : Insertion of the selection cassette in exon 2.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
Pathways
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB92506
Anti-STAT1 alpha antibody [EPYR2154]
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 alpha antibody [EPYR2154] (AB92506)](https://content.abcam.com/products/images/stat1-alpha-antibody-epyr2154-ab92506--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img449084.jpg)
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Product promise
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