Human CYP24A1 knockout A549 cell line
Be the first to review this product! Submit a review
|
(0 Publication)
CYP24A1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
1 25 dihydroxyvitamin D(3) 24 hydroxylase, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 24-OHase, 25-dihydroxyvitamin D(3) 24-hydroxylase, CP 24, CP24A_HUMAN, CYP 24, Cytochrome P450 24A1, Cytochrome P450 24A1 mitochondrial, Cytochrome P450 family 24, Cytochrome P450 family 24 subfamily A member 1, Cytochrome P450 family 24 subfamily A polypeptide 1, Cytochrome P450 subfamily XXIV, Cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase), Cytochrome P450-CC24, EC 1.14.13.n4, Exo mitochondrial protein, HCAI, HCINF1, MGC126273, MGC126274, P450 CC24, Vitamin D 24 hydroxylase, Vitamin D(3) 24-hydroxylase, mitochondrial
- NGS
Supplier Data
Next Generation Sequencing - Human CYP24A1 knockout A549 cell line (AB273735)
1 bp deletion after Lys134 of the WT protein
- NGS
Supplier Data
Next Generation Sequencing - Human CYP24A1 knockout A549 cell line (AB273735)
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift : 98%
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.
Pathways
CYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com