DGKD KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 14.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 14
Alternative names
130 kDa diacylglycerol kinase, DAG kinase delta, DGK-delta, DGKD_HUMAN, Diacylglycerol kinase delta, Diglyceride kinase delta, FLJ26930, KIAA0145
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DGKD KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 14.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 14
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- DGKD
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DGKD also known as Diacylglycerol kinase delta plays an essential role in lipid signaling by converting diacylglycerol (DAG) into phosphatidic acid (PA). This enzymatic activity impacts cellular processes such as growth and differentiation. The DGKD protein has a molecular mass of approximately 130 kDa and is expressed in various tissues with significant expression noted in the brain and muscle. The gene encoding DGKD is located on human chromosome 13 providing insights into its systemic importance.
- Biological function summary
DGKD participates in regulating intracellular signaling. DGKD modulates signal transduction by altering levels of second messengers like DAG. This regulation occurs because DGKD acts as a part of lipid kinase activities impacting membrane-bound complexes involved in signal pathways. These activities can play roles in neurotransmitter release and cellular responses to external stimuli signifying their critical involvement in maintaining cellular homeostasis.
- Pathways
DGKD plays a role in the phosphatidylinositol signaling system by interacting with and regulating proteins such as Protein Kinase C (PKC). This involvement means that it influences the kinase activity responsible for numerous cellular responses including cell growth apoptosis and metabolism. Additionally DGKD appears in the broader MAPK/ERK pathway indicating links with cellular proliferation and survival signals and suggesting its interconnectedness with a network of kinases and phosphatases.
- Associated diseases and disorders
DGKD abnormalities have connections with conditions like insulin resistance and neurological disorders. Studies point out its potential interaction with proteins like IRS1 in insulin signaling pathways where altered DGKD function might influence metabolic pathways leading to insulin resistance. Furthermore its significant presence in the brain suggests possible links to neurological conditions potentially through influencing synaptic signal regulation or cellular communication networks.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Cell Culture - Human DGKD knockout HeLa cell line (ab265991)
Representative images DGKD knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.
Sanger Sequencing - Human DGKD knockout HeLa cell line (ab265991)
Homozygous: 1 bp deletion in exon 14.
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