DPP4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
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Key facts
- Cell type
MCF7
- Species or organism
Human
- Tissue
Breast
- Form
Liquid
- Knockout validation
Next Generation Sequencing
Alternative names
ADA-binding protein, ADABP, ADCP-2, Adenosine deaminase complexing protein 2, CD 26, CD26 antigen, CD26 antigen 3, DPP IV, DPP IV estoenzyme, DPP4_HUMAN, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidyl peptidase, intestinal, Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2), Intestinal dipeptidyl peptidase, T-cell activation antigen CD26, TP 103
Recommended products
DPP4 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
Key facts
- Cell type
MCF7
- Species or organism
Human
- Tissue
Breast
- Form
Liquid
- Knockout validation
Next Generation Sequencing
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
DPP4
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Next Generation Sequencing
Cell culture
- Biosafety level
EU: 1 US: 1
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- Slow to trypsinise.
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5-7x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
MEM + 10% FBS + 0.01 mg/ml bovine insulin
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type MCF7 cell line (Human wild-type MCF7 cell line ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
- Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
- Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
- Associated diseases and disorders
DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
Product promise
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1 product image
Next Generation Sequencing - Human DPP4 knockout MCF7 cell line (ab289290)
86 bp deletion (allele 1) and 85 bp deletion (allele 2) in exon 9, CCDS2216.1
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