Human EGR1 knockout HeLa cell line
- Advanced Validation
- What is this?
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EGR1 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 100%.
View Alternative Names
AT225, EGR1_HUMAN, Early growth response 1, Early growth response protein 1, G0S30, KROX 24, NGFI-A, Nerve growth factor-induced clone A, Nerve growth factor-induced protein A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24
- WB
Lab
Western blot - Human EGR1 knockout HeLa cell line (AB274929)
Lanes 1 - 4:
Western blot - Anti-Egr1 antibody [EPR15916] (<a href='/en-us/products/primary-antibodies/egr1-antibody-epr15916-ab194357'>ab194357</a>) at 1/10000 dilution
Lanes 1 - 4:
Western blot - Anti-Egr1 antibody [EPR15916] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/egr1-antibody-epr15916-bsa-and-azide-free-ab232448'>ab232448</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa Vehicle Control TNF-a (0ng/mL, 1h) cell lysate at 20 µg
Lane 2:
Wild-type HeLa TNF-a (20ng/mL, 1h) cell lysate at 20 µg
Lane 3:
Western blot - Human EGR1 knockout HeLa cell line (ab274929)
Lane 3:
Western blot - Human EGR1 knockout HeLa cell lysate (ab274987)
Lane 3:
EGR1 knockout HeLa Vehicle Control TNF-a (0ng/mL, 1h) cell lysate at 20 µg
Lane 4:
EGR1 knockout HeLa TNF-a (20ng/mL, 1h) cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 73 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human EGR1 knockout HeLa cell line (AB274929)
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift : 100%
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab271142). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Egr1 acts as an immediate-early response gene. It gets rapidly upregulated in response to growth factors stress signals and other extracellular stimuli. As part of the cellular signaling networks Egr1 interacts with other transcription factors to control the expression of genes involved in cell differentiation proliferation and apoptosis. It does not typically form part of a protein complex but may work in concert with other signaling molecules to influence diverse cellular pathways and maintain cellular homeostasis.
Pathways
Egr1 is involved in the MAPK/ERK and PI3K/AKT signaling pathways both key to regulating cellular growth and survival. It functions as a bridging factor that links extracellular signals to transcriptional changes within the nucleus. In the MAPK/ERK pathway Egr1 works alongside proteins like Ras and MEK to promote early gene transcription in response to mitogenic signals. In the PI3K/AKT pathway Egr1 can influence downstream targets reinforcing the pathway's mechanisms in determining cell fate.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
![Western blot - Anti-Egr1 antibody [EPR15916] (AB194357)](https://content.abcam.com/products/images/egr1-antibody-epr15916-ab194357--western-blot-img418328.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com