Human EIF2AK1 (Heme-regulated inhibitor) knockout HEK-293T cell line
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- Sanger seq
Supplier Data
Sanger Sequencing - Human EIF2AK1 (Heme-regulated inhibitor) knockout HEK-293T cell line (AB266486)
Allele-2 : 2 bp insertion in exon 2.
- Sanger seq
Supplier Data
Sanger Sequencing - Human EIF2AK1 (Heme-regulated inhibitor) knockout HEK-293T cell line (AB266486)
Allele-1 : 1 bp insertion in exon2
Complementary Products
Reactivity data
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Heme-regulated inhibitor functions as an important part of the cellular stress response. It is not a standalone entity and often forms part of larger signaling networks as it interacts with eIF2α. In addition to its role in erythroid cells HRI responds to various stressors including oxidative stress and heat shock by modulating protein production which contributes to cellular adaptation mechanisms. Its activity helps maintain homeostasis by reducing protein synthesis when cells face adverse conditions.
Pathways
HRI significantly influences the integrated stress response (ISR) pathway and iron metabolism. HRI's activation through phosphorylation of eIF2α links it to related proteins such as activating transcription factor 4 (ATF4) which assists in adaptive gene expression changes under stress conditions. This action highlights HRI's involvement in pathways that regulate translation under various stress conditions maintaining proper cellular function and response to changes in environmental and internal conditions.
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com