Human ELAVL3 knockout U-87 MG cell line
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- NGS
Supplier Data
Next Generation Sequencing - Human ELAVL3 knockout U-87 MG cell line (AB306657)
103 bp deletion (allele 1) and 101 bp deletion (allele 2) after Asn23 of the WT protein
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Reactivity data
Product details
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
EMEM + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HuC significantly influences neuronal differentiation and development. It participates in controlling the stability and translation of mRNA transcripts in neurons. HuC often functions as part of larger RNA-protein complexes impacting the post-transcriptional modification of target RNAs. Through its ability to bind mRNA HuC promotes the expression of proteins essential for neuron function affecting how neurons develop and maintain their activity.
Pathways
HuC integrates into the post-transcriptional regulatory networks in neurons. It influences the MAPK signaling pathway which is important for cell proliferation and survival. Within these pathways HuC interacts with proteins such as HuD another member of the ELAV family to ensure proper mRNA processing and translational control. These interactions are vital for maintaining neuronal integrity and response to external stimuli.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
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