ELL2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 26 bp insertion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 26 bp insertion in exon 1
Alternative names
ELL related RNA polymerase II elongation factor, ELL2_HUMAN, Elongation factor RNA for polymerase II 2, Elongation factor RNA polymerase II 2, Elongation factor for RNA polymerase II 2, RNA polymerase II elongation factor ELL2
Recommended products
ELL2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 26 bp insertion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 26 bp insertion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- ELL2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELL2 also known as Eleven Nineteen Lysine-rich Leukemia 2 is a protein involved in transcriptional regulation. This protein has a molecular mass of approximately 76 kDa. ELL2 is expressed in various tissues including both lymphoid and non-lymphoid origins. It functions as an elongation factor for RNA polymerase II modulating the rate of transcription elongation by directly interacting with the transcription machinery.
- Biological function summary
ELL2 plays a significant role in RNA processing and maturation. It is a part of the ELL complex and works alongside other proteins that facilitate the transition from transcription initiation to elongation. By promoting RNA synthesis and elongation efficiency ELL2 is linked with gene expression regulation particularly within the adaptive immune system. This protein helps in optimizing the output of genes active in immune cells contributing to the functionality and differentiation of these cells.
- Pathways
ELL2 participates actively in the transcription regulation pathway and its interaction with RNA polymerase II. The protein influences gene expression regulation by enhancing transcriptional elongation. ELL2 is also involved in pathways associated with the immunoglobulin production process. It interacts with other proteins such as ELL1 and pTEFb which further coordinate the elongation steps of transcription thereby impacting overall gene expression in immune-related pathways.
- Associated diseases and disorders
ELL2 associates with multiple myeloma and immunodeficiency disorders. Its dysfunction can impact immunoglobulin transcription in plasma cells potentially leading to aberrant immunoglobulin production—an element in the pathogenesis of multiple myeloma. The association of ELL2 with RNA polymerase II and its partners like pTEFb sheds light on its role in these conditions as faulty regulation of transcription elongation can lead to abnormal cell proliferation and survival characteristic of certain cancers and immune disorders.
Product promise
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2 product images
Sanger Sequencing - Human ELL2 knockout HeLa cell line (ab265499)
Allele-2: 26 bp insertion in exon 1.
Sanger Sequencing - Human ELL2 knockout HeLa cell line (ab265499)
Allele-1: 1 bp insertion in exon 1.
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