EMC6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2
Alternative names
0610009E20Rik, 0610025L18Rik, MGC2963, OTTMUSP00000006414, OTTMUSP00000006415, RGD1309231, RP23-263M10.8, TMEM93, Transmembrane protein 93
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EMC6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 23 bp deletion in exon 2 and 94 bp deletion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- EMC6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transmembrane protein 93 also known as EMC6 functions as a membrane protein in intracellular transport and protein folding. EMC6 is part of the endoplasmic reticulum membrane protein complex (EMC) and has a molecular mass of approximately 26 kDa. It expresses widely in various tissues including brain heart liver and kidney suggesting a broad role in cellular processes.
- Biological function summary
This protein interacts with other components in the endoplasmic reticulum and is essential for proper folding and insertion of proteins into the ER membrane. EMC6 functions as part of a larger scaffolding complex that supports ER-associated degradation (ERAD) which is necessary for maintaining protein homeostasis. This complex plays an important role in quality control by removing misfolded proteins and EMC6 specifically contributes to the stabilization and function of this complex.
- Pathways
EMC6 has important roles in the ER stress response and autophagy pathways. The protein interacts with autophagy-related proteins including Beclin 1 facilitating the cellular recycling process. Through the ER stress response EMC6 participates in maintaining cellular health by assisting in protein refolding and degradation pathways linking its function to the action of other ER stress-related proteins like IRE1 and PERK.
- Associated diseases and disorders
EMC6 associates with conditions like neurodegenerative diseases and certain cancers. The protein's involvement in autophagy links it to Alzheimer's disease where accumulation of defective proteins occurs. Additionally disruptions in EMC6 function may contribute to oncogenesis by impairing protein quality control with its role in ERAD connecting it to the growth of cancerous cells. Altered expression or function of EMC6 can correlate with disease pathogenesis making it a point of interest for therapeutic research.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell line (ab265710)
Allele-2: 23 bp deletion in exon 2.
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell line (ab265710)
Allele-3: 1 bp deletion in exon 2.
Sanger Sequencing - Human EMC6 (Transmembrane protein 93) knockout HeLa cell line (ab265710)
Allele-1: 94 bp deletion in exon 2.
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