EMC8 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1
Alternative names
C16orf2, C16orf4, COX4 neighbor, COX4, neighbor of, COX4AL, CX4NB_HUMAN, ER membrane protein complex subunit 8, FAM158B, NOC4, Neighbor of COX4, Protein FAM158B, family with sequence similarity 158, member B
Recommended products
EMC8 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
EMC8
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein COX4NB also known as Cytochrome C Oxidase Subunit 4 Neighbor has a mass of around 20 kDa. It plays a mechanical role closely related to the function of its neighboring COX4 isoform proteins which are part of the cytochrome C oxidase complex. COX4NB is expressed in various human tissues including the heart and skeletal muscle suggesting its involvement in energy metabolism.
- Biological function summary
COX4NB participates in mitochondrial function. The protein integrates into the cytochrome C oxidase complex which is an important component of the electron transport chain in mitochondria. Through this involvement COX4NB contributes to cellular respiration and ATP production which maintain energy homeostasis in cells.
- Pathways
COX4NB is involved in key metabolic pathways. It is part of the oxidative phosphorylation pathway which is essential for the generation of ATP in aerobic organisms. In this process COX4NB works alongside proteins like COX4I1 and other subunits of the cytochrome C oxidase complex to facilitate efficient electron transfer and energy production.
- Associated diseases and disorders
COX4NB has associations with conditions tied to mitochondrial dysfunction. Its altered expression or mutation could relate to mitochondrial cardiomyopathy or hypertrophic cardiomyopathy conditions that affect heart muscle efficiency. Researchers are studying the link between COX4NB and proteins like COX4I1 as changes in these proteins could illuminate pathological mechanisms underpinning such mitochondrial diseases.
Product promise
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2 product images
Sanger Sequencing - Human EMC8 (COX4NB) knockout HeLa cell line (ab265851)
Allele-2: Insertion of the selection cassette in exon 1.
Sanger Sequencing - Human EMC8 (COX4NB) knockout HeLa cell line (ab265851)
Allele-1: 1 bp deletion in exon 1.
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