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EPCAM KO cell line available to order. KO validated by Immunocytochemistry, Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 99.58%.

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Images

Flow Cytometry - Human EPCAM knockout A-431 cell line (AB261902), expandable thumbnail
  • Western blot - Human EPCAM knockout A-431 cell line (AB261902), expandable thumbnail
  • Western blot - Human EPCAM knockout A-431 cell line (AB261902), expandable thumbnail
  • Next Generation Sequencing - Human EPCAM knockout A-431 cell line (AB261902), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Human EPCAM knockout A-431 cell line (AB261902), expandable thumbnail

Key facts

Cell type
A-431
Species or organism
Human
Tissue
Skin
Form
Liquid
Knockout validation
Immunocytochemistry, Next Generation Sequencing, Western blot
Mutation description
Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 99.58%

Alternative names

17 1A, 323/A3, AUA1, Adenocarcinoma-associated antigen, Antigen identified by monoclonal antibody AUA1, CD326, CD326 antigen, CO 17A, CO17 1A, Cell surface glycoprotein Trop-1, Cell surface glycoprotein Trop-2, DIAR5, EGP 2, EGP314, EGP40, EPCAM_HUMAN, ESA, EpCAM1, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, GA733 1, GA733-2, GP4, HNPCC8, Human epithelial glycoprotein 2, KS 1/4 antigen, KS1/4, KSA, Ly74, Lymphocyte antigen 74, M1S 1, M1S2, M4S1, MIC18, MK 1, Major gastrointestinal tumor-associated protein GA733-2, Membrane component chromosome 4 surface marker (35kD glycoprotein), Membrane component, chromosome 4, surface marker, Membrane component, chromosome 4, surface marker 1, Protein 289A, TACD1, TACSTD1, TROP1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1, gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein, hEGP 2, hEGP314, mEGP314

Recommended products

EPCAM KO cell line available to order. KO validated by Immunocytochemistry, Next Generation Sequencing, Western blot. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 99.58%.

Key facts

Cell type
A-431
Form
Liquid
Mutation description
Knockout achieved by CRISPR/Cas9 X = 2 bp deletion Frameshift: 99.58%
Disease
Epidermoid Carcinoma
Concentration
Loading...

Properties

Gene name
EPCAM
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Immunocytochemistry, Next Generation Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type A-431 cell line (ab263975). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

Associated diseases and disorders

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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