Human EPHX2 knockout K562 cell line
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- NGS
Lab
Next Generation Sequencing - Human EPHX2 knockout K562 cell line (AB301986)
79 bp deletion after Asn123 of the WT protein
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Subculture when cells reach 1x106 cells/mL.
Culture medium
IMDM + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Soluble epoxide hydrolase regulates the hydrolysis of epoxides which affects signaling molecules involved in inflammation and vascular tone. EPHX2 does not directly form a complex but functions within cellular environments to modulate lipid signaling pathways. Through its enzymatic action it converts epoxyeicosatrienoic acids (EETs) which helps to control vasodilation and anti-inflammatory responses.
Pathways
The enzyme soluble epoxide hydrolase integrates into the arachidonic acid metabolism pathway and the cytochrome P450 signaling pathway. In these pathways it interacts with metabolites like EETs influencing lipid signaling and inflammatory processes. Additionally its activity relates to proteins such as cyclooxygenase and lipoxygenase which participate in lipid mediator biosynthesis.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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