EPS8L2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
Alternative names
EPS8 like 2, EPS8-like protein 2, EPS8-related protein 2, EPS8R 2, ES8L2_HUMAN, Epidermal growth factor receptor kinase substrate 8-like protein 2, Epidermal growth factor receptor pathway substrate 8 like protein 2, Epidermal growth factor receptor pathway substrate 8-related protein 2, FLJ16738, FLJ21935, FLJ22171, MGC126530, MGC3088
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EPS8L2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- EPS8L2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EPS8L2 also known as Epidermal Growth Factor Receptor Substrate 8-Like Protein 2 plays a mechanical role in cellular signaling and cytoskeletal organization. This protein has a molecular mass of approximately 97 kDa. EPS8L2 expresses in various tissues including the brain liver and testis where it contributes to diverse cellular processes. Its structural components include actin-binding domains which allow interaction with the cytoskeleton and other signaling molecules.
- Biological function summary
EPS8L2 regulates actin cytoskeleton dynamics and signal transduction pathways. It is part of the EPS8 family of proteins and functions within a complex to mediate cell shape and movement. This protein acts as a scaffold that facilitates interactions among actin filaments and other proteins which influences cell migration and invasion. EPS8L2 modifies actin-related changes that are essential in dynamic cellular environments.
- Pathways
EPS8L2 integrates into the EGFR (Epidermal Growth Factor Receptor) signaling pathway influencing cellular growth and differentiation. In another role it participates in the MAPK/ERK pathway by interacting with components like GRB2 and SOS1. These pathways control various aspects of cell behavior impacting cell proliferation and survival. Related proteins such as EPS8 also share similar pathway interactions and have overlapping functions in cellular signaling.
- Associated diseases and disorders
EPS8L2 connects to cancer and neurodegenerative diseases. In cancer dysregulation of EGFR signaling involving EPS8L2 may contribute to tumor progression and metastasis. The protein's interactions influence cellular behaviors critical to cancer development. In neurodegenerative disorders alterations in cytoskeletal dynamics associated with EPS8L2 may exacerbate conditions like Alzheimer's Disease. This protein interacts with others like ApoE impacting pathways linked to neuronal health and degeneration.
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1 product image
Sanger Sequencing - Human EPS8L2 knockout HeLa cell line (ab265785)
Homozygous: Insertion of the selection cassette in exon 2.
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