Human ETS2 knockout THP1 cell line
- Advanced Validation
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ETS2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
C ets 2 protein, ETS2IT1, ETS2_HUMAN, Oncogene ETS 2, Protein C-ets-2, V ets avian erythroblastosis virus E26 oncogene homolog 2, V ets erythroblastosis virus E26 oncogene homolog 2, v ets avian erythroblastosis virus E2 oncogene homolog 2
- NGS
Supplier Data
Next Generation Sequencing - Human ETS2 knockout THP1 cell line (AB290239)
89 bp deletion after Leu69 of the WT protein.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for subculture for a number of days. Cells should be left as much as possible over this time and only subcultured when the cell density reaches 8x105 cells/mL.Small amounts of fresh media can be added until cell number/viability improves.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- Cells should be seeded at 2x105 - 3x105 cells/mL and subcultured when they have reached 8x105 cells/mL.
- It is not recommended to allow the cell density to exceed 1x106 cells/mL.
Culture medium
RPMI + 10% FBS + 0.05 mM beta-mercaptoethanol
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ETS2 protein functions in the regulation of genes that control critical cellular activities. The protein can form complexes with other transcription factors to modulate gene expression. It can interact with other proteins to influence cellular responses to external signals. As a transcription factor ETS2 also has a role in cellular differentiation and morphogenesis contributing to the development of multicellular organisms.
Pathways
ETS2 protein is involved in key signaling pathways such as the MAPK/ERK pathway and PI3K/AKT pathway. These pathways play essential roles in cell cycle regulation apoptosis and cell growth. ETS2 interacts with proteins like MAPK and AKT to transmit signals from the cell membrane to the nucleus. These interactions help coordinate responses to growth factors and stress signals influencing cellular outcomes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
Complementary Products
![Immunoprecipitation - Anti-ETS2 antibody [EPR22419] (AB219948)](https://content.abcam.com/products/images/ets2-antibody-epr22419-ab219948--immunoprecipitation-img4622.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com