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EXOC2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.

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Images

Western blot - Human EXOC2 knockout HeLa cell line (AB265839), expandable thumbnail
  • Sanger Sequencing - Human EXOC2 knockout HeLa cell line (AB265839), expandable thumbnail
  • Sanger Sequencing - Human EXOC2 knockout HeLa cell line (AB265839), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Form
Liquid
Knockout validation
Sanger Sequencing
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3

Alternative names

EXOC2_HUMAN, Exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5 like 1, SEC5L1, Sec5p

Recommended products

EXOC2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.

Key facts

Cell type
HeLa
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
EXOC2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The exocyst complex component 2 also known as EXOC2 or SEC5 is an important protein involved in vesicle trafficking. EXOC2 has an estimated mass of around 105 kDa. It is widely expressed in various tissues highlighting its broad significance in cellular processes. Its primary role is to participate in the tethering of secretory vesicles to specific sites on the plasma membrane aiding in processes like cell migration and growth.

Biological function summary

EXOC2 acts as a fundamental part of the exocyst complex which comprises eight proteins. This complex orchestrates vesicle targeting and tethering during exocytosis a process essential for maintaining cell surface area and membrane protein composition. Unlike other vesicle trafficking complexes the exocyst shows significant specificity for certain vesicles and localization contributing to the precision of membrane-associated functions.

Pathways

EXOC2 joins key pathways like the insulin signaling pathway and the regulation of actin cytoskeleton. In the insulin signaling pathway EXOC2 integrates with components such as the protein Rab11 to mediate glucose transporter vesicle trafficking impacting glucose uptake. In the regulation of the actin cytoskeleton it interacts with actin-related proteins to influence cell shape and motility.

Associated diseases and disorders

Connection with EXOC2 influences conditions such as cancer and diabetes. Abnormal EXOC2 expression links to various cancers often through disruptions in cellular trafficking and signal transduction. The interplay with diabetes arises partly through its role in insulin-mediated processes with proteins like Rab11 that affect glucose homeostasis. These associations highlight the potential for EXOC2 as a therapeutic target in these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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